Immunohistological features of visceral leishmaniasis in BALB/c mice
Immunohistological features of visceral leishmaniasis in BALB/c mice
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DOI：
10.1111/j.1365-3024.2006.00817.x
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发表时间：
2006-05-01
影响因子：
2.2
通讯作者：
Alonso, C
中科院分区：
文献类型：
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作者：
Carrión, J;Nieto, A;Alonso, C
据报道，针对鼠内脏利什曼病（VL）的疫苗提供的保护水平较低，VL研究的进展可能是由于缺乏适当的模型来研究保护性免疫。我们分析了 BALB/c 小鼠静脉注射婴儿利什曼原虫 10(3)、10(5) 和 10(6) 寄生虫后发生的免疫组织学特征。我们的结果表明，在所有情况下，寄生虫施用都会导致感染的建立并导致可量化的免疫组织学特征的发展，这些特征取决于接种量。这项研究表明，寄生虫攻击的差异导致 BALB/c 模型中与感染程度相关的一些参数的演变发生变化：抗利什曼原虫抗体水平、脾精氨酸酶活性上调、IFN-γ和 IL-10 之间的平衡、脾白髓中淋巴滤泡耗竭的程度以及肝肉芽肿的无效发展。此外，根据最初的感染性接种物，骨髓中是否存在寄生虫以及成熟和空型肉芽肿的数量是与保护相关的参数。我们认为，在该模型中，10(5) 种寄生虫的挑战应该对针对 VL 的疫苗研究有用。
It has been reported that the level of protection provided by vaccines against murine visceral leishmaniasis (VL) is low and that progress in research on VL may be due to the lack of appropriate models to study protective immunity. We have analysed the immunohistological features occurring in BALB/c mice after intravenous administration of 10(3), 10(5) and 10(6) parasites of Leishmania infantum. Our results show that in all cases parasite administration leads to the establishment of infection and to the development of quantifiable immunohistological features which are dependent on the inoculum size. This study demonstrates that differences in the parasite challenge result in changes in the evolution of some of the parameters associated with the degree of the infection in the BALB/c model: level of anti-Leishmania antibodies, up-regulation of spleen arginase activity, balance between IFN-gamma and IL-10, extent of lymphoid follicle depletion in the splenic white pulp and ineffective development of hepatic granulomas. Also, and depending on the initial infectious inoculum, the absence of parasites in the bone marrow and the number of mature and empty type granulomas were parameters associated with protection. We think that in this model a challenge of the order of 10(5) parasites should prove useful for vaccine studies against VL.