Protective effects of paeoniflorin on alveolar bone resorption and soft-tissue breakdown in experimental periodontitis
Protective effects of paeoniflorin on alveolar bone resorption and soft-tissue breakdown in experimental periodontitis
复制标题
芍药苷对实验性牙周炎牙槽骨吸收和软组织破坏的保护作用
DOI：
10.1111/jre.12305
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发表时间：
2016-04-01
影响因子：
3.5
通讯作者：
Li, C.
中科院分区：
文献类型：
--
作者：
Ni, J.;Yang, D.;Li, C.
背景与目的芍药苷是芍药中的主要活性成分，据报道具有明显的抗炎和免疫调节活性。本研究旨在探讨芍药苷对实验性牙周炎牙槽骨破坏和软组织破坏的保护作用。作为补充证据，我们评估了MMP-2、MMP-9、诱导型一氧化氮合酶(iNOS)和环氧合酶-2(COX-2)的相关表达。材料与方法28只大鼠随机分为以下四组：健康组；对照组；对照组。牙周炎组；牙周炎加用芍药苷（P30）30mg/kg组；牙周炎加60mg/kg芍药苷(P60)组。通过在下颌第一磨牙周围放置结扎线来诱发大鼠牙周炎。实验前后测量并记录每只大鼠的体重。使用微型计算机断层扫描评估骨吸收。通过组织学分析和天狼星红染色评估软组织破坏和胶原纤维降解。 Western blotting检测牙龈中MMP-2、MMP-9、iNOS、COX-2的表达水平。结果健康组牙周组织完整。牙周炎组牙槽骨水平明显降低，软组织炎症明显。芍药苷以剂量依赖性方式显着预防牙槽骨丢失和炎症浸润。 P30和P60组的胶原纤维分数显着高于牙周炎组。蛋白质印迹分析结果显示，30和60mg/kg的芍药苷均显着下调MMP-2、MMP-9、iNOS和COX-2的水平。结论本研究提供了第一个证据，表明芍药苷显着下调炎症浸润，防止实验性牙周炎中的牙槽骨丢失和软组织破坏。芍药苷的抗炎机制在一定程度上得到了其对 MMP-2、MMP-9、iNOS 和 COX-2 的抑制作用的进一步支持。
Background and ObjectivePaeoniflorin is the main active ingredient in Radix Paeoniae and has been reported to exhibit obvious anti-inflammatory and immune-regulatory activities. This study aimed to investigate the protective effects of paeoniflorin on alveolar bone destruction and soft-tissue breakdown in experimental periodontitis. As supplementary evidence, we evaluated the related expression of MMP-2, MMP-9, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2).Material and MethodsTwenty-eight rats were randomly placed into the following four groups: healthy group; periodontitis group; periodontitis plus 30mg/kg of paeoniflorin (P30) group; and periodontitis plus 60mg/kg of paeoniflorin (P60) group. Periodontitis was induced in rats by placing ligatures around the lower first molars. The body weight of each rat was measured and recorded before and after the experiment. Bone resorption was evaluated using micro-computed tomography. Soft-tissue destruction and collagen fiber degradation were assessed by histologic analysis and picrosirius red staining. The expression levels of MMP-2, MMP-9, iNOS and COX-2 in gingiva were detected by western blotting.ResultsPeriodontal tissues were intact in the healthy group. Alveolar bone level was significantly reduced, and evident inflammation of soft tissues was observed in the periodontitis group. Paeoniflorin significantly prevented alveolar bone loss and inflammatory infiltration in a dose-dependent manner. The collagen fiber fractions were significantly higher in the P30 and P60 groups than in the periodontitis group. The results of the western blot analyses revealed that both 30 and 60mg/kg of paeoniflorin significantly down-regulated the levels of MMP-2, MMP-9, iNOS and COX-2.ConclusionThis study provides the first evidence that paeoniflorin significantly down-regulates inflammatory infiltration and prevents alveolar bone loss and soft-tissue destruction in experimental periodontitis. The anti-inflammatory mechanism of paeoniflorin was further supported, in part, by its inhibitory effect on MMP-2, MMP-9, iNOS and COX-2.