EVIDENCE FOR HAPLOID EXPRESSION OF MOUSE TESTICULAR GENES
EVIDENCE FOR HAPLOID EXPRESSION OF MOUSE TESTICULAR GENES
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DOI：
10.1016/0014-4827(86)90465-9
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发表时间：
1986-05-01
影响因子：
3.7
通讯作者：
DISTEL, RJ
中科院分区：
文献类型：
--
作者：
HECHT, NB;BOWER, PA;DISTEL, RJ
从青春期前和性成熟的CD1小鼠的睾丸总RNA与几个小鼠睾丸cDNA探针的杂交结果表明，在22日龄小鼠的睾丸中首次检测到编码这两个精蛋白的mRNA，一个1.5kb的肌动蛋白序列，以及一个减数分裂后表达的620个核苷酸的mRNA。这些实验和其他研究用cDNA探针[1，2]分析分离的睾丸细胞类型群体的RNA制备，证明单倍体基因在哺乳动物的睾丸中表达。
Hybridization of RNA blots of total testicular RNA from prepubertal and sexually mature CD1 mice with several mouse testicular cDNA probes reveals that the mRNA encoding the two mouse protamines, an actin sequence of 1.5 kb, and a post-meiotically expressed 620 nucleotide mRNA are first detected in the testes of mice 22 days of age. These experiments and other studies analysing RNA preparations from isolated populations of testicular cell types with cDNA probes [1, 2] demonstrate that haploid gene expression occurs in the mammalian testis.