Formation of perfused, functional microvascular tubes in vitro
Formation of perfused, functional microvascular tubes in vitro
复制标题
DOI：
10.1016/j.mvr.2006.02.005
复制
发表时间：
2006-05-01
影响因子：
3.1
通讯作者：
Tien, Joe
中科院分区：
文献类型：
--
作者：
Chrobak, Kenneth M.;Potter, Daniel R.;Tien, Joe
本研究描述了体外三维微血管的形成、灌注和成熟过程。这些管道由人类内皮细胞的融合单层组成，这些单层排列在胶原凝胶内的开放圆柱形通道中。血管周围细胞可以直接包埋在血管内，也可以在内皮细胞生长融合后加入。管子横跨凝胶的整个5- 7mm范围；它们的直径最初为55 ~ 120 μ m，成熟后增加到75 ~ 150 μ m。内皮管在5天内表现出强大的屏障功能，抵抗白细胞的粘附，并通过破坏屏障和支持白细胞粘附对炎症刺激做出快速反应。这些管在细胞组织和功能上都类似于小静脉和“巨大”毛细血管，我们相信它们将在持续灌注下作为有用的体外炎症模型。(c) 2006爱思唯尔公司版权所有。
This work describes the formation, perfusion, and maturation of three-dimensional microvascular tubes in vitro. These tubes consisted of confluent monolayers of human endothelial cells that lined open, cylindrical channels within collagen gels. Perivascular cells could be directly embedded within the gets or added after endothelial cells grew to confluence. The tubes spanned the entire 5-7 mm extent of the gels; their diameters initially ranged from 55 to 120 mu m and increased to 75-150 mu m after maturation. Endothelial tubes displayed a strong barrier function over 5 days, resisted adhesion of leukocytes, and reacted quickly to inflammatory stimuli by breakdown of the barrier and support of leukocyte adhesion. These tubes resembled venules and "giant" capillaries in both their cellular organization and function, and we believe that they will serve as useful in vitro models of inflammation under constant perfusion. (c) 2006 Elsevier Inc. All rights reserved.