Phytosulphokine-α, a peptidyl plant growth factor, stimulates somatic embryogenesis in carrot
Phytosulphokine-α, a peptidyl plant growth factor, stimulates somatic embryogenesis in carrot
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植物磺因子-α，一种肽基植物生长因子，刺激胡萝卜体细胞胚胎发生
DOI：
10.1093/jxb/50.336.1123
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发表时间：
1999
影响因子：
6.9
通讯作者：
H. Kamada
中科院分区：
文献类型：
--
作者：
Toshihiro Kobayashi;Chang;Hidetoshi Hanai;Y. Matsubayashi;Y. Sakagami;H. Kamada
细胞在高细胞密度的培养基中可以在含有植物激素的培养基中增殖，但在低细胞密度的培养基中，细胞不能在含有植物激素的培养基中增殖。然而，低细胞密度的细胞激活肽作为植物生长因子。可以在条件培养基中生长（Stuart，它刺激芦笋和水稻的增殖，Street, 1969； Somers等人，1987；Birnberg等人，细胞，但还没有关于其影响的信息1988；Bellincampi和Morpurgo， 1987, 1989； Huang对植物形态发生的影响。）PSK-a对等人的影响，1990；Bellincampi et al., 1993)。在胡萝卜（Daucus carota L.）的体细胞胚胎发生中，迄今为止所鉴定的植物激素都无法替代。PSK-a，当添加到条件培养基诱导时。因此，它是体胚发生的假定培养基，增加了某些因素在条件培养基中积累的体胚数量。在低细胞[2‐5]PSK-a和酪氨酸磺酸酯（tyr - so3h）密度下培养的细胞中，化学物质类似并促进细胞分裂。Matsubayashi和Sakagami（1996）分离出的这两种植物硫素-a （PSK-a）作为阴性对照，对其他植物硫素-a （PSK-a）没有影响。另外，在体细胞胚发生过程中，芦笋叶肉细胞培养的增殖条件培养基也是PSK-a， PSK-a对芦笋叶肉细胞有增强作用。这些结果表明PSK-硫酸五肽H-Tyr(SO
Cells in culture with a high cell density can proliferate in medium that contains phytohormones, but cells at low Phytosulphokine-a (PSK-a) is the first chemically char- cell density cannot. However, cells at a low cell density acterized peptide that acts as a plant growth factor. can grow in the presence of conditioned medium (Stuart It stimulates the proliferation of asparagus and rice and Street, 1969; Somers et al., 1987; Birnberg et al., cells, but no information is yet available on its effects 1988; Bellincampi and Morpurgo, 1987, 1989; Huang on plant morphogenesis. The effects of PSK-a on et al., 1990; Bellincampi et al., 1993). None of the somatic embryogenesis in carrot (Daucus carota L.) phytohormones characterized to date is able to substitute were examined. PSK-a, when added to the induction for the conditioned medium. Thus, it was postulated medium for somatic embryogenesis, increased the that certain factors accumulate in conditioned medium number of somatic embryos. The chemical analogues and promote the division of cells cultured at low cell [2‐5]PSK-a and tyrosine sulphate ester (Tyr-SO 3 H), density. Matsubayashi and Sakagami (1996) isolated which have been used as negative controls in other phytosulphokine-a (PSK-a) as such a factor from the systems, had no effect. Moreover, the proliferation conditioned medium of a culture of mesophyll cells of of cells during somatic embryogenesis was also Asparagus oYcinalis L. PSK-a was determined to be a enhanced by PSK-a. These results indicate that PSK- sulphated pentapeptide: H-Tyr(SO