The contribution of TWIK-1 channels to astrocyte K(+) current is limited by retention in intracellular compartments.
The contribution of TWIK-1 channels to astrocyte K(+) current is limited by retention in intracellular compartments.
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DOI：
10.3389/fncel.2013.00246
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发表时间：
2013
影响因子：
5.3
通讯作者：
Zhou M
中科院分区：
文献类型：
--
作者：
Wang W;Putra A;Schools GP;Ma B;Chen H;Kaczmarek LK;Barhanin J;Lesage F;Zhou M
TWIK-1 two-pore domain K+ channels are expressed abundantly in astrocytes. In the present study, we examined the extent to which TWIK-1 contributes to the linear current-voltage (I–V) relationship (passive) K+ membrane conductance, a dominant electrophysiological feature of mature hippocampal astrocytes. Astrocytes from TWIK-1 knockout mice have a more negative resting potential than those from wild type animals and a reduction in both inward rectification and Cs+ permeability. Nevertheless, the overall whole-cell passive conductance is not altered significantly in TWIK-1 knockout astrocytes. The expression of Kir4.1 and TREK-1, two other major astrocytic K+ channels, or of other two-pore K+ channels is not altered in TWIK-1 knockout mice, suggesting that the mild effect of TWIK-1 knockout does not result from compensation by these channels. Fractionation experiments showed that TWIK-1 is primarily localized in intracellular cytoplasmic fractions (55%) and mildly hydrophobic internal compartment fractions (41%), with only 5% in fractions containing plasma membranes. Our study revealed that TWIK-1 proteins are mainly located in the intracellular compartments of hippocampal astrocyte under physiological condition, therefore a minimal contribution of TWIK-1 channels to whole-cell currents is likely attributable to a relatively low level presence of channels in the plasma membrane.
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影响因子：
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通讯作者：
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