A NOVEL MECHANISM FOR THE INITIATION OF TACARIBE ARENAVIRUS GENOME REPLICATION
A NOVEL MECHANISM FOR THE INITIATION OF TACARIBE ARENAVIRUS GENOME REPLICATION
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DOI：
10.1128/jvi.64.12.6196-6203.1990
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发表时间：
1990-12-01
影响因子：
5.4
通讯作者：
KOLAKOFSKY, D
中科院分区：
文献类型：
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作者：
GARCIN, D;KOLAKOFSKY, D
沙粒病毒基因组和反基因组 RNA 的末端高度保守，直接测定时，始终包含 3'' G（称为位置 +1）。然而，引物延伸至塔卡里布病毒基因组的 5'' 末端，反基因组延伸至位置 -1。当基因组和反基因组在分子间或分子内退火并用 RNase A 或 T1 处理时，杂合体的 5'' 末端似乎有一个未配对的 G。通过克隆 S 反基因组的 5'' 末端也发现了一个额外的 G，并且用加帽酶进行的研究检测了基因组和反基因组链 5'' 末端的 (p)ppG。提出了一种模型，其中基因组复制以 pppGpC 启动，以产生非模板化的额外 G。相反，N mRNA 5'' 末端的非模板化碱基（延伸至位置 -1 至 -5）被发现是有帽的，并且序列上也是异质的。
The ends of arenavirus genome and antigenome RNAs are highly conserved and where determined directly, always contain a 3'' G (referred to as position +1). However, primers extended to the 5'' ends of Tacaribe virus genomes and antigenomes extend to position -1. When genomes and antigenomes are annealed either inter or intramolecularly and treated with RNase A or T1, there appears to be a single unpaired G at the 5'' ends of the hybrids. A single extra G is also found by cloning the 5'' ends of S antigenomes, and studies with capping enzyme detect (p)ppG at the 5'' ends of genome and antigenome chains. A model is proposed in which genome replication initiates with pppGpC to create the nontemplated extra G. In contrast, the nontemplated bases at the 5'' ends of the N mRNAs, which extend to positions -1 to -5, were found to be capped and also heterogeneous in sequence.