INTRON SPLICING - A CONSERVED INTERNAL SIGNAL IN INTRONS OF ANIMAL PRE-MESSENGER RNAS
INTRON SPLICING - A CONSERVED INTERNAL SIGNAL IN INTRONS OF ANIMAL PRE-MESSENGER RNAS
复制标题
DOI：
10.1073/pnas.81.23.7417
复制
发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
NOON, WA
中科院分区：
文献类型：
--
作者：
KELLER, EB;NOON, WA
酵母前体mRNA内含子的剪接需要位于距3“内含子边界20-55个核苷酸的内部保守序列T-A-C-T-A-A-C。现在已经在各种动物基因的前mRNA内含子的相应内部区域中鉴定出仅在某些位置与该酵母信号不同的序列。使用搜索共有结构的同源物并计算每个同源物的匹配准确度的计算机程序来定位这些序列。列出了在海胆、小鼠、大鼠和人类基因内含子中发现的信号，并给出了每个物种的共识。果蝇和鸡和鸭的信号的共识也被发现。积累的证据表明，这些内部信号需要在动物中剪接，然后讨论。还注意到，小核RNA U2的单链区域包含与所提出的哺乳动物内部信号和相邻信号互补的序列。图形 **。在3“内含子边界处。U2核糖核蛋白在内含子剪接的作用，因此建议。
Splicing of introns of yeast pre-mRNA requires an internal conserved sequence T-A-C-T-A-A-C that is located 20-55 nucleotides from the 3'' intron boundary. Sequences differing only in certain positions from this yeast signal have now been identified in the corresponding internal region of pre-mRNA introns of a variety of animal genes. A computer program that searches for homologues to a consensus structure and calculates the accurary of match of each homologue is used to locate these sequences. The signals found by this search in introns of sea urchin, mouse, rat and human genes are listed and the consensus for each species is given. The consensus found for Drosophila and chicken and duck signals is also given. The accumulating evidence that these internal signals are required for splicing in animals is then discussed. It is also noted that a single-stranded region of small nuclear RNA U2 contains sequences complementary both to the proposed mammalian internal signal and to the neighboring .**GRAPHIC**. at the 3'' intron boundary. A role for U2 ribonucleoprotein in intron splicing is thus suggested.