Expression of constitutive endothelial and inducible nitric oxide synthase in the sciatic nerve of Lewis rats with experimental autoimmune neuritis
Expression of constitutive endothelial and inducible nitric oxide synthase in the sciatic nerve of Lewis rats with experimental autoimmune neuritis
复制标题
DOI：
10.1016/s0165-5728(02)00058-9
复制
发表时间：
2002-05-01
影响因子：
3.3
通讯作者：
Shin, T
中科院分区：
文献类型：
--
作者：
Lee, Y;Shin, T
本研究检测了患有实验性自身免疫性神经炎 (EAN) 的 Lewis 大鼠坐骨神经中组成型内皮一氧化氮合酶 (eNOS) 和诱导型一氧化氮合酶 (iNOS) 的表达。 Western blot分析显示，大鼠坐骨神经中eNOS和iNOS表达在EAN高峰期显着增加，但随后下降。在正常大鼠坐骨神经中仅发现了极少量的这些酶。免疫组织化学研究表明，在 EAN 的高峰期，血管内皮细胞和雪旺细胞中的 eNOS 增加，但炎症细胞中的 eNOS 没有增加。然而，iNOS 主要存在于坐骨神经 EAN 病变的炎症巨噬细胞中。这些发现表明，根据周围神经系统自身免疫性疾病的阶段，eNOS 和 iNOS 表达的增加可能与 EAN 诱导阶段有害作用的产生或 EAN 麻痹的恢复有关。 (C) 2002 Elsevier Science B.V. 保留所有权利。
This study examined the expression of constitutive endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) in the sciatic nerve of Lewis rats with experimental autoimmune neuritis (EAN). Western blot analysis showed that both eNOS and iNOS expressions in the sciatic nerves of rats increased significantly during the peak stage of EAN, but declined thereafter. Only minimal amounts of these enzymes were identified in normal rat sciatic nerves. Immunohistochemical studies showed that eNOS was increased in vascular endothelial cells and Schwann cells, but not in inflammatory cells, during the peak stage of EAN. However, iNOS was found mainly in inflammatory macrophages in sciatic nerve EAN lesions. These findings suggest that, depending on the stage of peripheral nervous system autoimmune disease, the increased expressions of both eNOS and iNOS might be involved in either the production of detrimental effects during the induction stage of EAN or in the recovery from EAN paralysis. (C) 2002 Elsevier Science B.V. All rights reserved.