METHODOLOGICAL REFINEMENTS TO THE MOUSE PAW FORMALIN TEST - AN ANIMAL-MODEL OF TONIC PAIN
METHODOLOGICAL REFINEMENTS TO THE MOUSE PAW FORMALIN TEST - AN ANIMAL-MODEL OF TONIC PAIN
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DOI：
10.1016/0160-5402(88)90078-2
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发表时间：
1988-09-01
期刊：
影响因子：
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通讯作者：
COWAN, A
中科院分区：
文献类型：
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作者：
MURRAY, CW;PORRECA, F;COWAN, A
福尔马林强直性疼痛测试最近已适用于小鼠。我们观察到此过程存在两个问题。首先，与载体对照组相比，在后爪注射稀释福尔马林的大多数小鼠表现出显着的伤害性反应（舔爪），包括活动峰值，远远超出了建议的观察期结束；其次，反应出现了令人无法接受的巨大差异。我们报告了消除这些困难的两项修改：a）将福尔马林注射后的观察期延长至 20-45 分钟，b）在将福尔马林注射到爪子之前用乙醚轻轻麻醉小鼠。通过评估吗啡、喷他佐辛和佐美拉克（镇痛 A50 值分别为 2.1、23.8 和 >100 mg/kg，s.c.）来测试修改后的方案。给轻度麻醉的小鼠鞘内或脑室内注射生理盐水，不会干扰伤害性行为；这一发现扩大了测试的适用性和有用性。总的来说，这些改进显着改善了小鼠强直性疼痛的福尔马林模型。
The formalin tonic pain test has recently been adapted for use in mice. We have observed two problems with this procedure. First, compared to a vehicle control group, the majority of mice injected in a hind paw with dilute formalin exhibited a significant nociceptive response (paw licking), including peaks of activity, well beyond the end of the recommended observation period; and, second, an unacceptably wide variation in response occurred. We report two modifications that eliminate these difficulties: a) extending the observation period to 20-45 min postformalin injection, and b) lightly anesthetizing the mice with ether before injection of formalin into the paw. The modified protocol was tested by evaluating morphine, pentazocine and zomepirac (analgesic A50 values were 2.1, 23.8, and >100 mg/kg, s.c., respectively). An intrathecal or intracerebroventricular injection of saline, given to the lightly anesthetized mouse, did not interfere with the nociceptive behavior; this finding widens the applicability and usefulness of the test. Collectively, these refinements significantly improve the mouse formalin model of tonic pain.