Comparison of the neuropeptide Y receptor in the rat brain and intestine.
Comparison of the neuropeptide Y receptor in the rat brain and intestine.
复制标题
大鼠大脑和肠道中神经肽 Y 受体的比较。
DOI：
10.1111/j.1749-6632.1990.tb48921.x
复制
发表时间：
1990
影响因子：
5.2
通讯作者：
Nguyen,TD
中科院分区：
文献类型：
--
作者：
Taylor,IL;Mannon,PJ;Heintz,GG;Kaiser,LM;Nguyen,TD
神经肽Y（NPY）与胰多肽（PP）和肽YY（PYY）一起构成一个结构相关的肽家族，它们均含有36个氨基酸，并具有相似的三级结构。'-4 NPY在中枢、外周和肠神经中具有神经递质和神经调节功能。NPY中枢给药可增加食物摄入量，产生低血压、呼吸缓慢和脑电图同步。 6 并改变昼夜节律^。^ 应用于安装在尤斯室中的肠粘膜的 NPY 抑制离子传输，EC 为 10-30 nM；*。"它抑制跨壁电位和短路电流，增加粘膜-浆膜 Na+ 和 C1-通量，并减少浆膜-粘膜 C1-通量。*-"只有当 NPY 添加到肠上皮的浆膜侧（但不是管腔侧）。这些观察结果与 NPY 定位于肠神经系统内的内在神经相关，这些神经的末端非常接近肠上皮细胞的侧基底区域。我们使用了最近描述的方法，该方法允许分离不受管腔刷状缘膜（BBM）污染的肠浆膜侧基底膜（LBM）、细胞内内质网（ER）和高尔基囊泡，以证明NPY优先与肠上皮细胞的浆膜LBM结合。此外，我们将放射性标记的 NPY 与其肠道受体交联，并将在 SDS 聚丙烯酰胺凝胶电泳上观察到的复合物与交联脑受体后观察到的模式进行比较。
Neuropeptide Y (NPY), together with pancreatic polypeptide (PP) and peptide YY (PYY), constitute a family of structurally related peptides all of which contain 36 amino acids and have a similar tertiary structure.'-4 NPY has neurotransmitter and neuromodulator functions in the central, peripheral and enteric nervous Central administration of NPY increases food intake, s produces hypotension, bradypnea and EEG synchronization. 6 and shifts circadian rhythm^.^ NPY applied to intestinal mucosa mounted in Ussing chambers inhibits ion transport with an EC,, of 10-30 nM;*." it inhibits transmural electrical potential and short circuit current, increases mucosal-toserosal Na+ and C1-fluxes, and reduces serosal-to-mucosal C1-fluxes.*-" This inhibitory action is only demonstrable when NPY is added to the serosal (but not luminal) side of the intestinal epithelium. These observations correlate with the localization of NPY to intrinsic nerves within the enteric nervous system that end in close proximity to the laterobasal region of the intestinal epithelial cell. We have used a recently described method that allowed isolation of intestinal serosal latero-basal membranes (LBM) free from contamination with luminal brush border membranes (BBM), and intracellular endoplasmic reticulum (ER) and Golgi vesiclesi2 to demonstrate that NPY binds preferentially to the serosal LBM of the enterocyte. In addition we have cross-linked radiolabelled NPY to its intestinal receptor, and compared the resulting complexes observed on SDS-polyacrylamide gel electrophoresis with the pattern observed after cross-linking the brain receptor.