Simultaneous quantification of imidacloprid and its metabolites in tissues of mice upon chronic low-dose administration of imidacloprid
Simultaneous quantification of imidacloprid and its metabolites in tissues of mice upon chronic low-dose administration of imidacloprid
复制标题
长期低剂量施用吡虫啉后小鼠组织中吡虫啉及其代谢物的同时定量
DOI：
10.1016/j.chroma.2021.462350
复制
发表时间：
2021
影响因子：
4.1
通讯作者：
Ishizuka Mayumi
中科院分区：
文献类型：
--
作者：
Nimako Collins;Ikenaka Yoshinori;Akoto Osei;Fujioka Kazutoshi;Taira Kumiko;Arizono Koji;Kato Keisuke;Takahashi Keisuke;Nakayama Shouta M.M.;Ichise Takahiro;Ishizuka Mayumi
本研究旨在（i）开发一种灵敏的方法，用于同时检测和定量组织样本中的吡虫啉（IMI）及其7种代谢物，并（ii）确定IMI化合物在C57 BL/6 J雄性小鼠组织中的生物分布;通过粉末饲料暴露于0.6 mg/kg bw/天的IMI（IMI无明显不良影响水平的10%）24周后。我们成功地开发了一种方法，该方法具有准确性（大多数化合物的回收率≥ 70%），灵敏度（所有检测化合物的LOD ≤ 0.47 ng/mL和LOQ ≤ 1.43 ng/mL，R2≥ 0.99）和精密度（RSD ≤ 20%），可用于血液和各种组织基质中IMI及其7种代谢物的常规分析。经生物分布分析，在小鼠体内检测到IMI及其5种代谢产物。脑、睾丸、肺、肾、腹股沟白色脂肪组织和性腺白色脂肪组织主要积累IMI，血液和肠系膜白色脂肪组织主要积累IMI-烯烃;肝脏主要积累去硝基IMI;胰腺主要积累4-羟基IMI。在睾丸、脑、肺和肾中，去硝基-异戊基-IMI和去硝基-IMI代谢产物的组织-血液浓度比≥ 1.0。发现六种检测到的IMI化合物（106种IMI化合物）的累积水平以降序排列：血液>睾丸>脑>肾>肺> iWAT > gWAT > mWAT >肝>胰腺。总之，这项研究提供了必要的数据，需要有效的机制阐明与长期暴露于IMI的哺乳动物物种的化合物特定的不良后果。
This study aimed to (i) develop a sensitive method for simultaneous detection and quantification of imidacloprid (IMI) and seven of its metabolites in tissue specimens, and to (ii) determine the biodistribution of the IMI compounds in tissues of C57BL/6J male mice; after exposure to 0.6 mg/kg bw/day of IMI (10% of no observable adverse effect level of IMI) through a powdered diet for 24 weeks. We successfully developed a method which was accurate (recoveries were ≥ 70% for most compounds), sensitive (LODs ≤ 0.47 ng/mL and LOQs ≤ 1.43 ng/mL were recorded for all detected compounds,R2≥ 0.99) and precise (RSDs ≤ 20%) for routine analysis of IMI and seven of its metabolites in blood and various tissue matrices. After bio-distributional analysis, IMI and five of its metabolites were detected in mice. Brain, testis, lung, kidney, inguinal white adipose tissue and gonadal white adipose tissue mainly accumulated IMI, blood and mesenteric white adipose tissue mainly accumulated IMI-olefin; liver mainly accumulated desnitro-IMI; pancreas predominately accumulated 4-hydroxy-IMI. The desnitro-dehydro-IMI and the desnitro-IMI metabolites recorded tissue-blood concentration ratios ≥ 1.0 for testis, brain, lung and kidney. The cumulative levels of the six detected IMI compounds (Σ6 IMI compounds) were found in the decreasing order: blood > testis > brain > kidney > lung > iWAT > gWAT > mWAT > liver > pancreas. Altogether, this study provided essential data needed for effective mechanistic elucidation of compound-specific adverse outcomes associated with chronic exposures to IMI in mammalian species.