Characterization of type 1 and type 2 cytokine production profile in physiologic and pathologic human pregnancy
Characterization of type 1 and type 2 cytokine production profile in physiologic and pathologic human pregnancy
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DOI：
10.1046/j.1365-2249.1996.d01-809.x
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发表时间：
1996-10-01
影响因子：
4.6
通讯作者：
Clerici, M
中科院分区：
文献类型：
--
作者：
Marzi, M;Vigano, A;Clerici, M
分析了50名孕妇和31名年龄和性别匹配的非妊娠对照的外周血单个核细胞（PBMC）的抗原和促分裂原刺激的细胞因子产生，以确定细胞因子产生的变化是否发生在正常和病理性人类妊娠期间。在3个月期间连续入组的妊娠女性在进入研究时正经历正常、非病理性妊娠，并接受超声检查以确定准确的妊娠周数和胎儿活力。50例妊娠中有40例（80%）在生理上终止妊娠，产下正常婴儿。在5/50（10%）名妇女中观察到自然流产，5名妇女产下小于胎龄儿（SGA）的新生儿。在正常妊娠中观察到IL-2和干扰素-γ（IFN-γ）的产生减少，伴随着IL-4和IL-10的产生增加，在妊娠晚期存在最低量的IL-2和IFN-γ以及最高量的IL-4和IL-10。统计学上显著增加IL-2和IFN-γ的产生和减少IL-10的产生表征病理性妊娠，并将其与正常妊娠区分开来。这些初步数据表明，2型细胞因子谱可能与正常人妊娠相关，而缺乏显性2型细胞因子谱可能表明病理性妊娠。
Antigen- and mitogen-stimulated cytokine production by peripheral blood mononuclear cells (PBMC) of 50 pregnant women and 31 age- and sex-matched non-pregnant controls were analysed to determine whether changes in cytokine production occur during normal and pathologic human gestation. The pregnant women, consecutively enrolled during a 3-month period, were undergoing a normal, non-pathologic pregnancy at the time of entry into the study, and underwent ultrasound examination to ascertain the exact week of pregnancy and the vitality of the fetus. Forty of the 50 pregnancies (80%) terminated physiologically with the birth of normal babies. Spontaneous abortions were observed in 5/50 (10%) women, and five women gave birth to newborns small For gestational age (SGA). A decrease in the production of IL-2 and interferon-gamma (IFN-gamma) accompanied by an increase in production of IL-4 and IL-10, was observed in normal pregnancy, with the lowest quantities of IL-2 and IFN-gamma and the highest quantities of IL-4 and IL-10 present in the third trimester of pregnancy. Statistically significant increased production of both IL-2 and IFN-gamma and reduced production of IL-10 characterized pathologic pregnancies and distinguished them from normal pregnancies. These preliminary data suggest that a type 2 cytokine profile may be associated with normal human pregnancy, whereas the lack of a dominant type 2 cytokine profile may be indicative of a pathologic pregnancy.