Detection of water-borne E. coli O157 using the integrating waveguide biosensor.
Detection of water-borne E. coli O157 using the integrating waveguide biosensor.
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DOI：
10.1016/j.bios.2005.01.005
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发表时间：
2005-10
影响因子：
12.6
通讯作者：
P. Zhu;D. Shelton;J. Karns;A. Sundaram;Shuhong Li;P. Amstutz;Cha-Mei Tang
中科院分区：
文献类型：
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作者：
P. Zhu;D. Shelton;J. Karns;A. Sundaram;Shuhong Li;P. Amstutz;Cha-Mei Tang
大肠杆菌O 157：H7是肠出血性大肠杆菌最常见的血清型。大肠杆菌（EHEC）是全球范围内许多食源性和水源性感染的原因。介绍了一种用于检测水中大肠杆菌的集成波导生物传感器。coliO 157，基于在玻璃毛细管波导内进行的荧光夹心免疫测定。捕获的E. coliO 157细胞，随后进行定量实时PCR以评估生物传感器捕获效率。还记录了毛细管波导中的体外微生物生长。该生物传感器允许定量检测少至10个细胞/毛细管（0.075毫升体积），并可与细胞扩增，PCR和微阵列技术结合使用，以积极识别病原体。
Escherichia coli O157:H7, the most common serotype of enterohemorrhagic E. coli (EHEC), is responsible for numerous food-borne and water-borne infections worldwide. An integrating waveguide biosensor is described for the detection of water-borne E. coli O157, based on a fluorescent sandwich immunoassay performed inside a glass capillary waveguide. The genomic DNA of captured E. coli O157 cells was extracted and quantitative real-time PCR subsequently performed to assess biosensor-capture efficiency. In vitro microbial growth in capillary waveguide is also documented. The biosensor allows for quantitative detection of as few as 10 cells per capillary (0.075ml volume) and can be used in conjunction with cell amplification, PCR and microarray technologies to positively identify a pathogen.