Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells
Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells
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DOI：
10.1016/j.biomaterials.2006.06.017
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发表时间：
2006-12-01
期刊：
影响因子：
14
通讯作者：
Mao, Hai-Quan
中科院分区：
文献类型：
--
作者：
Chua, Kian-Ngiap;Chai, Chou;Mao, Hai-Quan
造血干/祖细胞(HSPC)与其细胞外基质成分的相互作用是HSPC存活、增殖和分化信号调控的重要组成部分。我们假设底物形态线索和生化线索都可以与细胞因子补充协同作用，以促进HSPC的体外扩增。在这项研究中，我们比较了人脐血CD34(+)细胞在未经修饰、羟化、羧化和胺化的纳米纤维和薄膜上的体外扩增。10天的扩增结果显示，胺化纳米纤维网和薄膜支持CD34(+)CD45(+)细胞的扩增(分别是195倍和178倍)，而组织培养聚苯乙烯(50倍，p<；0.05)。特别是，与胺化薄膜相比，胺化纳米纤维网支持更高的细胞粘附度和HSPC百分比。扫描电子显微镜成像显示，离散的细胞集落在增殖，并与胺化纳米纤维相互作用。这项研究强调了生物材料方法在体外影响HSPC增殖和分化的潜力。(C)2006爱思唯尔有限公司。保留所有权利。
Interaction between hematopoietic stem/progenitor cells (HSPCs) and their extra cellular matrix components is an integral part of the signaling control for HSPC survival, proliferation and differentiation. We hypothesized that both substrate topographical cues and biochemical cues could act synergistically with cytokine supplementation to improve ex vivo expansion of HSPCs. In this study, we compared the ex vivo expansion of human umbilical cord blood CD34(+) cells on unmodified, hydroxylated, carboxylated and aminated nanofibers and films. Results from 10-day expansion cultures showed that aminated nanofiber mesh and film were most efficient in supporting the expansion of the CD34(+)CD45(+) cells (195-fold and 178-fold, respectively), as compared to tissue culture polystyrene (50-fold, p < 0.05). In particular, aminated nanofiber meshes supported a higher degree of cell adhesion and percentage of HSPCs, as compared to aminated films. SEM imaging revealed the discrete colonies of cells proliferating and interacting with the aminated nanofibers. This study highlights the potential of a biomaterials approach to influence the proliferation and differentiation of HSPCs ex vivo. (c) 2006 Elsevier Ltd. All rights reserved.