Recovery of Medium-Chain-Length Poly(3-Hydroxyalkanoates) from Pseudomonas putida KT2440 by NaOH Digestion
Recovery of Medium-Chain-Length Poly(3-Hydroxyalkanoates) from Pseudomonas putida KT2440 by NaOH Digestion
复制标题
通过 NaOH 消化从恶臭假单胞菌 KT2440 中回收中链长度聚（3-羟基链烷酸酯）
DOI：
10.1089/ees.2013.0305
复制
发表时间：
2014
影响因子：
1.8
通讯作者：
A. RamsayJuliana
中科院分区：
文献类型：
--
作者：
JiangXuan Jade;A. RamsayBruce;A. RamsayJuliana
摘要建立了一种利用NaOH消化法从恶臭假单胞菌KT 2440中回收中链聚羟基脂肪酸酯（MCL-PHA）的方法。PHA纯度受几个因素影响，包括NaOH浓度、温度、消化时间和处理循环次数。在含有66%PHA的70 g/L生物质用0.2N NaOH在22°C±1°C处理2小时或用0.1N在80°C处理15分钟后，获得约85%的PHA纯度。然而，0.2N NaOH在22°C±1°C下连续处理2 h，然后0.1N NaOH在80°C±1°C下连续处理15 min，产生具有94.7%的更高纯度和88%的回收率的PHA。在这些条件下，消化对PHA分子量的影响可以忽略不计。进一步用溶菌酶处理得到纯度为98.6%。这是第一个研究表明，MCL-PHA的密度和结晶度低于其他类别的PHA可以有效地回收碱消化。该方法是一种比目前工业生产更环保、安全风险更低的替代方法。
Abstract A method for the recovery of medium-chain-length polyhydroxyalkanoate (MCL-PHA) from Pseudomonas putida KT2440 was developed using NaOH digestion. PHA purity was affected by several factors, including NaOH concentration, temperature, digestion time, and number of treatment cycles. PHA purity of about 85% was attained after 70 g/L biomass containing 66% PHA was treated with 0.2 N NaOH at 22°C±1°C for 2 h or with 0.1 N at 80°C for 15 min. However, sequential treatment of 0.2 N NaOH at 22°C±1°C for 2 h followed by 0.1 N NaOH at 80°C±1°C for 15 min produced PHA with a higher purity of 94.7% and a recovery efficiency of 88%. Under these conditions, digestion had a negligible effect on PHA molecular weight. Further treatment with lysozyme gave a purity of 98.6%. This is the first study to demonstrate that MCL-PHA of lower density and crystallinity than other classes of PHAs can be effectively recovered by alkaline digestion. This method is a greener alternative and lower safety risk than current indust...