Selective Uptake of Small RNA Molecules in the Virion of Murine Gammaherpesvirus 68
Selective Uptake of Small RNA Molecules in the Virion of Murine Gammaherpesvirus 68
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DOI：
10.1128/jvi.02303-08
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发表时间：
2009-03-01
影响因子：
5.4
通讯作者：
Dutia, Bernadette M.
中科院分区：
文献类型：
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作者：
Cliffe, Anna R.;Nash, Anthony A.;Dutia, Bernadette M.
非编码RNA是许多疱疹病毒基因组的特征。它们包括microRNA，其功能是深入研究的主题，此外还有更长的RNA分子，如EB病毒编码的RNA和疱疹病毒saimiri U RNA，它们已经知道了一段时间，但其功能仍然没有得到很好的定义。鼠γ疱疹病毒68（MHV-68）编码8种功能未知的病毒tRNA样分子（vtRNA）。研究vtRNA的表达动力学，我们观察到它们在病毒感染后直接存在。这强烈表明vtRNA是病毒体结构的一部分，这通过在各种纯化的RNA酶处理的制剂中检测到它们而得到证实。尽管在MHV-68病毒粒子内也检测到病毒和细胞mRNA，但存在的主要RNA种类是长度约70个核苷酸的小RNA。有趣的是，病毒mRNA的掺入与感染细胞中的相对丰度无关，因为在病毒体中发现了以低丰度存在的M11 mRNA。缺乏编码vtRNA的基因的MHV-76也在病毒体中掺入小RNA分子，表明病毒体成熟需要这些分子。在生产性感染的细胞中，vtRNA主要位于细胞质内，尽管它们也表现出核染色的球状图案。它们在细胞质中的存在与病毒颗粒成熟前与病毒体组分的相互作用一致。这些发现的意义病毒粒子的结构和功能进行了讨论。
Noncoding RNAs are a feature of many herpesvirus genomes. They include microRNAs, whose function is the subject of intense investigation, in addition to longer RNA molecules such as the Epstein-Barr virus-encoded RNAs and herpesvirus saimiri U RNAs, which have been known for some time but whose function is still not well defined. Murine gammaherpesvirus 68 (MHV-68) encodes eight viral tRNA-like molecules (vtRNAs) of unknown function. Investigating the kinetics of expression of the vtRNAs, we observed that they were present directly after infection with the virus. This strongly suggested that vtRNAs were part of the virion structure, which was confirmed by their detection within various purified, RNase-treated preparations. Although both viral and cellular mRNAs were also detected within the MHV-68 virion, the major RNA species present were small RNAs of around 70 nucleotides in length. Interestingly, incorporation of viral mRNA was not related to the relative abundance in infected cells, as M11 mRNA, which is present at low abundance, was found in virions. MHV-76, which lacks the genes encoding the vtRNAs, also incorporated small RNA molecules within the virion, suggesting a requirement for these molecules for virion maturation. In productively infected cells the vtRNAs localized predominantly within the cytoplasm, although they also exhibited a globular pattern of nuclear staining. Their presence in the cytoplasm is consistent with interaction with virion components prior to maturation of virus particles. The significance of these findings for virion architecture and function is discussed.