Human umbilical cord blood CD34+ cell purification with high yield of early progenitors.
Human umbilical cord blood CD34+ cell purification with high yield of early progenitors.
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DOI：
10.1089/scd.1.1993.2.275
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
Hatzfeld, J
中科院分区：
文献类型：
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作者：
Cardoso, A A;Li, M L;Hatzfeld, J
人脐带血CD 34+细胞采用两步程序纯化，即通过消除大豆凝集素结合细胞和用CD 34单克隆抗体进行阳性淘选。分离的级分是88-97%纯的⑶ 34+细胞。当比较从CD 34（+）纯化组分中回收的细胞数量和在初始单核细胞组分中检测到的CD 34+细胞数量时，获得48.5%的产率。通过流式细胞术，我们观察到未回收的CD 34+细胞是那些具有较低表达的CD 34抗原的细胞，因此是更成熟的细胞。还观察到CFU-GEMM祖细胞的高回收率（73.9%）。这些数据表明纯化CD 34+脐带血细胞用于临床应用的可能性，特别是用于脐带血库。
Human umbilical cord blood CD34+ cells were purified using a two-step procedure by elimination of the soybean agglutinin-binding cells and by a positive panning selection with a CD34 monoclonal antibody. The isolated fraction was 88-97% pure CD34+ cells. A yield of 48.5% was obtained when comparing the number of cells recovered in the CD34(+)-purified fraction and the number of CD34+ cells detected in the initial mononuclear cell fraction. By flow cytometry, we observed that the CD34+ cells that were not recovered were those that had the lower expression of CD34 antigen and were therefore the more mature cells. A high recovery of CFU-GEMM progenitors (73.9%) was also observed. These data suggest the possibility of purifying CD34+ umbilical cord blood cells for clinical applications, in particular for umbilical cord blood banking.