INVITRO EFFECTS OF DIHYDROTESTOSTERONE ON GRANULOSA-CELL PRODUCTION OF ESTROGEN AND PROGESTERONE
INVITRO EFFECTS OF DIHYDROTESTOSTERONE ON GRANULOSA-CELL PRODUCTION OF ESTROGEN AND PROGESTERONE
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DOI：
10.1016/0039-128x(91)90044-v
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发表时间：
1991-05-01
期刊：
影响因子：
2.7
通讯作者：
MILLS, TM
中科院分区：
文献类型：
--
作者：
CONWAY, BA;MILLS, TM
为探讨雄激素对卵泡发育的直接影响，正常未成熟雌性大鼠在PMSG后0、12、24、36h分别给予8IU PMSG(0h)和4次注射溶剂或双氢睾酮(DHT)，剂量为1 mg/kg体重。从小卵泡(200-mU-m)、中卵泡(200-400 mU-m)和大卵泡(>；400-mU-m)分离颗粒细胞，加入或不加入0.5-mU-MDHT，体外培养48小时，测定培养液中孕酮和雌激素的含量。结果表明，无论卵泡大小如何，DHT都能增加所有颗粒细胞中孕酮的积聚。然而，DHT抑制了来自不同大小卵泡的颗粒细胞中雌激素的积累，这种抑制作用因体内雄激素暴露的时间而异。小卵泡颗粒细胞体内雌激素积累受到抑制，而中、大卵泡颗粒细胞经DHT处理后雌激素积累受到抑制。综上所述，体内和/或体外DHT治疗的实验结果表明，无论卵泡大小如何，雄激素都能促进颗粒细胞孕酮的合成。然而，颗粒细胞的雌激素积累依赖于卵泡的大小和DHT暴露的时间。
To investigate the direct effects of androgens on follicle development, intact, immature female rats were given 8 IU PMSG (0 hour) and four injections of either vehicle or dihydrotestosterone (DHT), 1 mg/kg body weight, at 0, 12, 24, and 36 hours after PMSG. Granulosa cells from small (< 200-mu-m), medium (200 to 400-mu-m), and large (> 400-mu-m) follicles were isolated and cultured in the presence or absence of 0.5-mu-M DHT in vitro for 48 hours, and the medium was assayed for progesterone and estrogen. Results show that DHT caused an increase in progesterone accumulation in all granulosa cells, regardless of follicle size. However, DHT inhibited estrogen accumulation in granulosa cells from different-size follicles and the inhibition varied depending on the duration of androgen exposure in vivo. The inhibition of estrogen accumulation was seen in granulosa cells from small follicles without prior exposure to DHT in vivo, while an inhibition of estrogen accumulation was seen in granulosa cells from medium and large follicles exposed to DHT treatment in vivo. Taken together, the results of experiments with in vivo and/or in vitro DHT treatment show that the androgen increases granulosa cell progesterone synthesis regardless of follicle size. However, the estrogen accumulation by granulosa cell is dependent on follicle size and duration of DHT exposure.