Gold Nanoparticle Arrangement on Viral Particles through Carbohydrate Recognition: A Non-Cross-Linking Approach to Optical Virus Detection
Gold Nanoparticle Arrangement on Viral Particles through Carbohydrate Recognition: A Non-Cross-Linking Approach to Optical Virus Detection
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DOI：
10.1021/bc900255x
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发表时间：
2009-10-01
影响因子：
4.7
通讯作者：
Ijiro, Kuniharu
中科院分区：
文献类型：
--
作者：
Niikura, Kenichi;Nagakawa, Keita;Ijiro, Kuniharu
我们提出了一种基于金纳米粒子在病毒表面空间组装的光学病毒检测新方法。由于JC病毒样颗粒（VLPs）包含一个与唾液酸结合的重复病毒衣壳蛋白，因此唾液酸连接的金颗粒与VLPs的结合使得金颗粒在VLP表面的空间排列成为可能。由于相邻金粒子之间的等离子体耦合，这种结构在吸收光谱中产生红移，从而导致光学病毒检测系统的构建。我们的系统不是依赖于VLPs和Au粒子的简单交联，而是依赖于覆盖VLPs整个表面的有序Au结构，并且可以应用于利用动物病毒固有配体识别的病毒检测系统。
We propose a new approach to optical virus detection, based on the spatial assembly of gold nanoparticles on the surface of viruses. Since JC virus-like particles (VLPs) comprise a repeating viral capsid protein that binds to sialic acid, the conjugation of sialic acid-linked Au particles with VLPs enables the spatial arrangement of Au particles on the VLP surface. This structure produced a red shift in the absorption spectrum due to plasmon coupling between adjacent Au particles, leading to the, construction of an optical virus detection system. Our system depends not on the simple cross-linking of VLPs and Au particles, but on an ordered Au structure covering the entire surface of the VLPs and can be applied to virus detection systems using the inherent ligand recognition of animal viruses.