The potential of bifidobacteria as a source of natural folate
The potential of bifidobacteria as a source of natural folate
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DOI：
10.1111/j.1365-2672.2012.05261.x
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发表时间：
2012-05-01
影响因子：
4
通讯作者：
Andlid, T.
中科院分区：
文献类型：
--
作者：
D'Aimmo, M. R.;Mattarelli, P.;Andlid, T.
目的：对19株双歧杆菌在合成的无叶酸和复合叶酸培养基中的主要叶酸形式组成进行了筛选。方法和结果：采用高效液相色谱法分析了从细菌生物量中提取的去结合叶酸。大多数菌株的总叶酸含量超过4000 μ g/100 g干物质（DM）。在链状双歧杆菌ATCC 27539中发现了9295 μ g/100 g DM的最高值，而在动物双歧杆菌亚种中发现了最低值。动物ATCC 25527，每100 g DM含220 μ g。10株生长在合成的无叶酸培养基（FFM），显示叶酸自养和建议叶酸营养缺陷的其余9个。在自养型菌株中，与更复杂的含叶酸培养基相比，在FFM中发现了一贯较高的叶酸水平，这表明在生长因子存在下对叶酸的需求减少，否则需要叶酸进行合成。总叶酸、5-CH_3-H（4）叶酸和H（4）叶酸的含量与菌株有关。结论：双歧杆菌叶酸含量和组成是动态的，具有菌株特异性，并依赖于培养基。适当选择生长条件可以导致每细胞单位生物量中含有高水平的叶酸。研究的意义和影响：这表明某些双歧杆菌可能有助于叶酸摄入，无论是直接在食物中，例如发酵乳制品，还是在肠道中作为营养叶酸的益生菌或天然微生物群的一部分。
Aims: To screen 19 strains of bifidobacteria for main folate forms composition in synthetic folate-free and complex folate-containing media.Methods and Results: HPLC was used to analyse deconjugated folates extracted from bacterial biomass. Most strains had a total folate content above 4000 mu g per 100 g dry matter (DM). The highest value of 9295 mu g per 100 g DM was found in Bifidobacterium catenulatum ATCC 27539 and the lowest in Bifidobacterium animalis ssp. animalis ATCC 25527 containing 220 mu g per 100 g DM. Ten strains grew in a synthetic folate-free medium (FFM), showing folate autotrophy and suggesting folate auxotrophy of the remaining nine. In the autotrophic strains, a consistently higher folate level was found in FFM as compared to a more complex folate-containing medium, suggesting reduced requirements for folates in the presence of growth factors otherwise requiring folates for synthesis. The contents of total folate, 5-CH3-H(4)folate and H(4)folate were strain dependent. 5-CH3-H(4)folate dominated in most strains.Conclusions: Our results show that bifidobacteria folate content and composition is dynamic, is strain specific and depends on the medium. Suitable selection of the growth conditions can result in high levels of folate per cell unit biomass.Significance and Impact of the Study: This suggests that certain bifidobacteria may contribute to the folate intake, either directly in foods, such as fermented dairy products, or in the intestine as folate-trophic probiotics or part of the natural microbiota.