Characterisation of a non-haemolytic enterotoxin complex from Bacillus cereus isolated after a foodborne outbreak
Characterisation of a non-haemolytic enterotoxin complex from Bacillus cereus isolated after a foodborne outbreak
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DOI：
10.1016/0378-1097(96)00208-x
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发表时间：
1996-08-01
影响因子：
2.1
通讯作者：
Granum, PE
中科院分区：
文献类型：
--
作者：
Lund, T;Granum, PE
从1995年挪威发生的一次大规模食物中毒中分离出三种肠毒性成分。在三个不同的柱上通过色谱法纯化组分。发现分别为39、45和105 kDa的三种蛋白质是最大细胞毒性所必需的。测定了39和45 kDa蛋白的氨基酸N-末端序列。45 kDa蛋白与Tecra检测的主要抗原相同，39 kDa蛋白与B溶血素BL的L（1）蛋白有一定的相似性。[咒语]此外，这三种毒性组分都被报道用于检测来自B的肠毒素的多克隆抗血清所识别。蜡状。这些蛋白质不同于溶血素BL的B-和L（2）-组分，以前认为是主要的毒力因子，并且没有可检测的溶血活性。
Three enterotoxic components have been isolated from a strain of Bacillus cereus which was involved in a large food poisoning outbreak in Norway in 1995. The components were purified by chromatography on three different columns. Three proteins of 39, 45 and 105 kDa, respectively, were found to be necessary for maximum cytotoxicity. The amino acid N-terminal sequences of the 39 and 45 kDa proteins were determined. The 45 kDa component was the same protein as the main antigen detected in the Bacillus Diarrhoeal Enterotoxin Visual Immunoassay (Tecra), The 39 kDa protein showed some similarity to the L(1) protein of haemolysin BL from B. cei eus. Furthermore, the three toxic components were all recognised by a polyclonal antiserum reported to detect enterotoxin from B. cereus. The proteins were different from the B- and L(2)-components of haemolysin BL, previously suggested to be a primary virulence factor, and had no detectable haemolytic activity.