Replication of DNA by nuclei isolated from soybean suspension cultures.
Replication of DNA by nuclei isolated from soybean suspension cultures.
复制标题
通过从大豆悬浮培养物中分离的细胞核进行 DNA 复制。
作者：
R. Román
以悬浮培养的大豆细胞为材料，对其细胞核DNA复制进行了研究。分离过程包括原生质体的制备、非离子洗涤剂的裂解和细胞核的纯化。这些细胞核合成低分子量DNA，并将这些片段连接成中等分子量的DNA。荧光脱氧尿嘧啶核苷同步化和紫外光照射实验表明，在分离的细胞核中复制的特征与分离的细胞的复制特征很好地相关。
DNA replication was studied in nuclei isolated from soybean cells grown in suspension culture. The isolation procedure involved the preparation of protoplasts, their lysis with a nonionic detergent and purification of nuclei. These nuclei synthesized low molecular weight DNA and joined these fragments into DNA of intermediate molecular weight. The characteristics of replication in isolated nuclei correlated well with those of the cells from which they were isolated, as shown by fluorodeoxyuridine synchronization and ultraviolet irradiation experiments.