IMPROVED DETECTION LIMITS FOR ELECTROSPRAY-IONIZATION ON A MAGNETIC-SECTOR MASS-SPECTROMETER BY USING AN ARRAY DETECTOR
IMPROVED DETECTION LIMITS FOR ELECTROSPRAY-IONIZATION ON A MAGNETIC-SECTOR MASS-SPECTROMETER BY USING AN ARRAY DETECTOR
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DOI：
10.1016/1044-0305(94)85033-x
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发表时间：
1994-03-01
影响因子：
3.2
通讯作者：
MUSSELMAN, BD
中科院分区：
文献类型：
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作者：
CODY, RB;TAMURA, J;MUSSELMAN, BD
阵列检测进行了比较，点检测的解决方案，鸡蛋白溶菌酶，马肌红蛋白，和泛素分析的电喷雾电离与磁扇区质谱仪。通过使用阵列检测器系统分析的样品的检测限比通过在同一质谱仪上使用点检测器可以实现的检测限低至少10倍。对于500阿莫尔L/穆尔的鸡蛋清溶菌酶溶液，蛋白质的最小可检测量对应于约2：1的信号背景比。然而，对于分析相对纯的蛋白质或简单混合物的稀释溶液，最终的实际样品浓度似乎在10-100 fmol/穆尔范围内。
Array detection was compared with point detection for solutions of hen egg-white lysozyme, equine myoglobin, and ubiquitin analyzed by electrospray ionization with a magnetic sector mass spectrometer. The detection limits for samples analyzed by using the array detector system were at least 10 times lower than could be achieved by using a point detector on the same mass spectrometer. The minimum detectable quantity of protein corresponded to a signal-to-background ratio of approximately 2:1 for a 500 amol/muL solution of hen egg-white lysozyme. However, the ultimate practical sample concentrations appeared to be in the 10-100 fmol/muL range for the analysis of dilute solutions of relatively pure proteins or simple mixtures.