Selection of genomic target RNAs by iterative screening.
Selection of genomic target RNAs by iterative screening.
复制标题
通过迭代筛选选择基因组靶RNA。
DOI：
10.1016/s0968-0896(01)00029-3
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发表时间：
2001
影响因子：
3.5
通讯作者：
McKeown,M
中科院分区：
文献类型：
--
作者：
Brunel,C;Ehresmann,B;Ehresmann,C;McKeown,M
已知越来越多的蛋白质通过RNA结合发挥其调节或生物学功能。在某些情况下，遗传相互作用使我们能够推断出RNA定向调控的候选靶点，但在许多其他情况下，潜在调控靶点的鉴定是有问题的。我们已经开发了一种体外生物化学筛选，SETIS（通过迭代S筛选的靶RNA的SE选择），其允许筛选基因组的主要部分以鉴定RNA结合蛋白的潜在靶。
A growing number of proteins are known to exert their regulatory or biological functions via RNA binding. In some cases genetic interactions allow us to infer candidate targets for RNA directed regulation, but in many other cases identification of potential regulatory targets is problematic. We have developed an in vitro biochemical screen, SETIS (SE lection of <> T arget RNAs by I terative S creening) that allows screening of a major portion of the genome for identification of potential targets for RNA binding proteins.