Gene expression profiling of selenophosphate synthetase 2 knockdown in Drosophila melanogaster
Gene expression profiling of selenophosphate synthetase 2 knockdown in Drosophila melanogaster
复制标题
果蝇中硒磷酸合成酶 2 敲低的基因表达谱
DOI：
10.1039/c5mt00134j
复制
发表时间：
2016
期刊：
影响因子：
3.4
通讯作者：
Zhang Yan
中科院分区：
文献类型：
--
作者：
Li Gaopeng;Liu Liying;Li Ping;Chen Luonan;Song Haiyun;Zhang Yan
Selenium (Se) is an important trace element for many organisms and is incorporated into selenoproteins as selenocysteine (Sec). In eukaryotes, selenophosphate synthetase SPS2 is essential for Sec biosynthesis. In recent years, genetic disruptions of both Sec biosynthesis genes and selenoprotein genes have been investigated in different animal models, which provide important clues for understanding the Se metabolism and function in these organisms. However, a systematic study on the knockdown of SPS2 has not been performedin vivo. Herein, we conducted microarray experiments to study the transcriptome of fruit flies with knockdown of SPS2 in larval and adult stages. Several hundred differentially expressed genes were identified in each stage. In spite that the expression levels of other Sec biosynthesis genes and selenoprotein genes were not significantly changed, it is possible that selenoprotein translation might be reduced without impacting the mRNA level. Functional enrichment and network-based analyses revealed that although different sets of differentially expressed genes were obtained in each stage, they were both significantly enriched in the carbohydrate metabolism and redox processes. Furthermore, protein–protein interaction (PPI)-based network clustering analysis implied that several hub genes detected in the top modules, such as Nimrod C1 and regucalcin, could be considered as key regulators that are responsible for the complex responses caused by SPS2 knockdown. Overall, our data provide new insights into the relationship between Se utilization and several fundamental cellular processes as well as diseases.
