The impact of Chlamydia trachomatis infection on sperm parameters and male fertility: A comprehensive study
The impact of Chlamydia trachomatis infection on sperm parameters and male fertility: A comprehensive study
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DOI：
10.1177/0956462417735245
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发表时间：
2018-04-01
影响因子：
1.4
通讯作者：
Meybodi, A. Mohseni
中科院分区：
文献类型：
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作者：
Moazenchi, M.;Totonchi, M.;Meybodi, A. Mohseni
沙眼衣原体(CT)是一种专性细胞内细菌，需要活细胞进行复制。感染CT的男性中有一半是没有症状的。CT感染可以在夫妇中持续长达四年，并影响他们的生育能力。男性的衣原体感染是传播给女性的蓄水池，可导致尿道炎、精子DNA损伤和急性附睾炎。对1080例精子图正常和异常的不育症患者的精液标本进行CT检测。用酶联免疫吸附试验检测患者精浆中抗CT-IgA的存在。用特异性引物进行分子检测，也证实了CT感染。为探讨CT感染对精子DNA断裂指数(DFI)的影响，采用流式细胞术对40例CT感染患者和20例CT阴性对照的精子染色质结构进行了分析。在1080例精子参数较差的患者中，CT诊断155例(14.3%)，其中精液异常患者占11%，无精液异常患者占26%。病例组DFI显著高于对照组(P<0.05)。
Chlamydia trachomatis (CT), an obligate intracellular bacterium, requires living cells to replicate. Half of men infected with CT are asymptomatic. CT infection can persist for up to four years within couples and affect their fertility. Chlamydia infection in men acts as a reservoir for transmission to women and can cause urinary tract inflammation, sperm DNA damage, and acute epididymitis. Semen samples from 1080 subfertile patients with normal and abnormal spermograms were examined to detect the presence of CT. An ELISA test was used to detect the presence of anti-CT IgA in these patients' seminal plasma. CT infection was also confirmed by molecular investigation using specific primers. In order to evaluate the effect of CT infections on the DNA Fragmentation Index (DFI), 40 CT-infected cases and 20 CT-negative controls were analyzed by a Sperm Chromatin Structure Assay using flow cytometry. Among 1080 patients with poor sperm parameters, 155 (14.3%) patients were diagnosed with CT, 11% among those with semen abnormalities and 26% among those without semen abnormalities patients. The DFI was statistically higher in cases than in controls (p