Microtiter plate assays for inhibition of human, drug-metabolizing cytochromes P450

Microtiter plate assays for inhibition of human, drug-metabolizing cytochromes P450
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DOI:
10.1006/abio.1997.2145
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发表时间:
1997-05-15
影响因子:
2.9
通讯作者:
Penman, BW
Penman, BW
中科院分区:
生物学4区
文献类型:
--
作者:
Crespi, CL;Miller, VP;Penman, BW

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(Sigma,目录号 510-A)并在 ELISA 读数装置中在 420 nm 处读取。在我们手中,这种方法似乎比 Schulze Charles L. Crespi、1 Vaughn P. Miller 等人 (7) 基于在 Bruce W. Penman 醋酸盐缓冲液中对细胞进行机械破碎的方法受到的限制要小得多。
(Sigma, Cat. No. 510-A) and read at 420 nm in a ELISA reader apparatus. In our hands, this method appears much less constraining than the procedure of Schulze Charles L. Crespi, 1 Vaughn P. Miller, et al.(7) based on mechanical desintegration of cells in and Bruce W. Penman acetate buffer.