Comparative study on hemostatic, cytotoxic and hemolytic activities of different species of Paris L.
Comparative study on hemostatic, cytotoxic and hemolytic activities of different species of Paris L.
复制标题
DOI：
10.1016/j.jep.2012.05.065
复制
发表时间：
2012-08-01
影响因子：
5.4
通讯作者：
Liu, Changxiao
中科院分区：
文献类型：
--
作者：
Liu, Zhen;Li, Nan;Liu, Changxiao
民族药理学相关性：重楼的根茎。 yannanensis 或 P. polyphylla var.重楼作为中药，自古以来在民间具有清热解毒、消肿止痛的功效。重楼应用的日益广泛导致野生动物资源的短缺。在这里，我们比较了重楼属其他物种的主要活动，以寻找替代植物。 材料和方法：从中国三个省收集了 6 个物种（云南重楼、德拉瓦伊重楼、Fargesii 重楼、巴山重楼、小重楼和拟藏重楼），比较了不同测定方法的止血活性、细胞毒活性和溶血活性。结果：对于止血活性，除重楼外，所有植物均具有止血活性。射箭能显着缩短尾部出血时间和凝血时间（P < 0.05）。为了进一步的机制研究，他们减少了凝血酶原时间（PT）和活化部分凝血活酶时间（APTT），但对凝血酶时间（TT）没有显着影响。 P.fargesii 变种。箭竹表现出与重楼相似的细胞毒性。云南山药（IC50：分别为 18.21 和 15.73 mu g/mL）。以HD50作为溶血活性指标。 P. delavayi 变种。地拉瓦伊和巴山重楼最晚具有该活性，其值分别为3.027和1.222 mg/mL。结论：重楼属不同种具有不同的活性。巴黎德拉瓦伊变种地拉瓦伊和重楼可以作为止血药物和重楼的资源。箭竹作为抗肿瘤药物。 (C) 2012 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: The rhizoma of Paris polyphylla var. yannanensis or P. polyphylla var. chinensis called Rhizoma Paridis as a traditional Chinese medicine has an effect of heat-clearing and detoxicating, detumescence and acesodyne in folk for a long time. The increasing application of Rhizoma Paridis resulted in the shortage of wildlife resources. Here, we compared the major activities of other species of genus Paris to find the replacement plants.Materials and methods: Six species (P. polyphylla var. yunnanensis, P. delavayi var. delavayi, P. fargesii var. Fargesii, P. bashanensis Wang et Tang, P. polyphyllar var. minora, and P. polyphylla var. pseudothibetical) were collected from three Provinces in China, and compared the hemostatic, cytotoxic and hemolytic activities by different assays.Results: For the hemostatic activity, all the plants except Paris fargesii var. Fargesii could significantly shorten the tail bleeding time and blood clotting time (P < 0.05). For further mechanism study, they reduced the prothrombin time (PT) and activated partial thromboplastin time (APTT), but they had no significant effect on thrombin time (TT). P. fargesii var. Fargesii showed the similar cytotoxicity to P. polyphylla var. yunnanensis (IC50: 18.21 and 15.73 mu g/mL, respectively). HD50 was used as the index of hemolytic activity. P. delavayi var. delavayi and P. bashanensis Wang et Tang were the last to have this activity as the values were 3.027 and 1.222 mg/mL.Conclusions: The different species of genus Paris have different activities. Paris delavayi var. delavayi and Paris bashanensis Wang et Tang could be used as the resources of hemostatic drugs and P. fargesii var. Fargesii as the antitumor medicine. (C) 2012 Elsevier Ireland Ltd. All rights reserved.