Determination of hyperoside and isoquercitrin in rat plasma by membrane-protected micro-solid-phase extraction with high-performance liquid chromatography.
Determination of hyperoside and isoquercitrin in rat plasma by membrane-protected micro-solid-phase extraction with high-performance liquid chromatography.
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DOI：
10.1002/jssc.201100867
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发表时间：
2012-02
影响因子：
3.1
通讯作者：
X. Yin;Yong-ming Luo;Jian-jiang Fu;You-quan Zhong;Qing-shan Liu
中科院分区：
文献类型：
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作者：
X. Yin;Yong-ming Luo;Jian-jiang Fu;You-quan Zhong;Qing-shan Liu
建立了一种基于膜保护分子印迹聚合物的微固相萃取法提取大鼠血浆中金丝桃苷和异槲皮苷的方法。将合成的金丝桃苷分子印迹聚合物包封在多孔聚醚砜膜中进行萃取。对微固相萃取的吸附剂材料、膜类型、萃取时间和解吸条件进行了优化。在最佳条件下，金丝桃苷和异槲皮苷的线性范围分别为1 ~ 120 μg/mL，相关系数分别为0.998和0.999。金丝桃苷和异槲皮苷的方法检出限分别为0.24和0.22 μg/mL。与传统的固相萃取、液液萃取和蛋白质沉淀等方法相比，该方法具有操作简单、提取效率高、环境友好等优点，特别适用于复杂的生物样品。
A novel method, micro-solid-phase extraction based on membrane-protected molecularly imprinted polymer, was developed to extract hyperoside and isoquercitrin in rat plasma. Synthesized hyperoside MIPs were packed in a porous polyether sulfone membrane envelope to perform extraction. The parameters sorbent materials, membrane types, extraction time and desorption conditions were optimized for micro-solid-phase extraction. Under the optimal conditions, correlation coefficients, 0.998 and 0.999, were obtained for hyperoside and isoquercitrin, respectively, with the linear range between 1 and 120 μg/mL. The absolute extraction recoveries from 84.5 to 89.3% were found. The method detection limits of hyperoside and isoquercitrin were 0.24 and 0.22 μg/mL, respectively. Compared with traditional methods, solid-phase extraction, liquid-liquid extraction and protein precipitation, the developed method was simple, highly efficient for extraction, environmentally friendly, and particularly suitable for complex biological samples.