Proliferation of microbodies in Saccharomyces cerevisiae
Proliferation of microbodies in Saccharomyces cerevisiae
复制标题
酿酒酵母中微体的增殖
作者：
M. Veenhuis;M. Mateblowski;W. Kunau;W. Harder
研究了不同生长条件对酿酒酵母微体发育的影响。在所有的转移实验中，以指数生长期的细胞在葡萄糖上为接种体，对不同的酿酒酵母菌株进行了研究。包括连续切片在内的电子显微镜显示，这些细胞通常含有一到四个定位于细胞壁附近的小微体，其特征是存在过氧化氢酶。将这些葡萄糖培养的细胞转移到添加了已知的在其他酵母中诱导微体增殖的各种化合物的培养基中--即尿酸、烷基化胺、氨基酸、C2-化合物，如乙醇或醋酸酯，在存在或不存在诱导氧自由基形成的化合物的情况下，没有导致观察到的微体轮廓的显著变化。然而，在细胞转移到含有油酸的培养液中后，观察到显著的微体增殖，并与β氧化酶的活性诱导有关。此外，过氧化氢酶和异柠檬酸裂解酶的表达水平也有所提高。动力学实验表明，这些微体是从接种细胞中最初存在的微体发展而来的。在薄片中，偶尔可以观察到多达14个微体轮廓，通常以小簇的形式出现。它们的最终体积分数达到细胞质体积的8-10%。
The development of microbodies in the yeast Saccharomyces cerevisiae was studied in response to different conditions of growth. Various strains of S. cerevisiae were investigated, using cells from the exponential growth phase on glucose as an inocullum in all transfer experiments. Electron microscopy, including serial sectioning, revealed that these cells generally contained one to four small microbodies which were localized in the vicinity of the cell wall and characterized by the presence of catalase. Transfer of these glucose‐grown cells into media supplemented with various compounds known to induce microbody proliferation in other yeasts—i.e. uric acid, alkylated amines, amino acids, C2‐compounds such as ethanol or acetate, in the presence or absence of compounds that induce oxygen radical formation—did not result in a significant change in the number of microbody profiles observed. Marked microbody proliferation was, however, observed after a shift of cells into media containing oleic acid and was associated with the induction of activities of β‐oxidation enzymes. In addition, catalase and isocitrate lyase were present in enhanced levels. Kinetic experiments suggested that these microbodies developed from those originally present in the inoculum cells. In thin sections up to 14 microbody profiles were occasionally observed, often present in small clusters. Their ultimate volume fraction amounted to 8–10% of the cytoplasmic volume.
大肠杆菌脂肪酸氧化复合物的大亚基是一种多功能多肽。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yang,SY;Schulz,H
通讯作者：
Schulz,H