An Hydroalcoholic Chamomile Extract Modulates Inflammatory and Immune Response in HT29 Cells and Isolated Rat Colon
An Hydroalcoholic Chamomile Extract Modulates Inflammatory and Immune Response in HT29 Cells and Isolated Rat Colon
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DOI：
10.1002/ptr.5655
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发表时间：
2016-09-01
影响因子：
7.2
通讯作者：
Brunetti, Luigi
中科院分区：
文献类型：
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作者：
Menghini, Luigi;Ferrante, Claudio;Brunetti, Luigi
炎症性肠病（IBD）是以结肠粘膜或消化道的任何部分的破坏和溃疡为特征的慢性病症（克罗恩病）。抗氧化/抗炎草药提取物补充剂可能代表了一种对比IBD的创新方法。临床试验证明了含有洋甘菊的天然配方对胃肠道疾病患者的疗效。这与洋甘菊的抗氧化和抗炎特性是一致的，尽管是部分一致的。本研究的目的是探讨洋甘菊提取物的可能的保护作用，对人类结肠直肠腺癌HT 29细胞，和大鼠结肠标本处理脂多糖（LPS）诱导的炎症刺激，一个良好的急性溃疡性结肠炎模型。在这种情况下，评估了炎症和脂质过氧化的不同生物标志物的活性，例如ROS、髓过氧化物酶（MPO）、5-羟色胺（5-HT）、前列腺素（PG）E-2、8-异前列腺素（8-iso-PG）F-2a、NF-kB、肿瘤坏死因子（TNF）α和白细胞介素（IL）-6。我们发现洋甘菊提取物在减少炎症刺激后MPO、5-HT、IL-6、NF-kB、TNF α、PGE（2）和8-iso-PGF（2 α）的产生方面与柳氮磺胺吡啶（2 mg/ml）一样有效。所观察到的调节作用支持使用洋甘菊补充剂作为预防和管理人类溃疡性结肠炎的有前景的药理学工具的合理性。版权所有（C）2016约翰威利父子有限公司
Inflammatory bowel diseases (IBDs) are chronic disorders characterized by disruption and ulceration of the colonic mucosa or of any part of the digestive tract (Crohn's disease). Antioxidant/anti-inflammatory herbal extract supplementation could represent an innovative approach to contrast IBDs. Clinical trials demonstrated the efficacy of natural formulas, containing chamomile, in patients with gastrointestinal disorders. This is consistent, albeit in part, with the antioxidant and anti-inflammatory properties of chamomile. The aim of the present study was to explore the possible protective role of a chamomile extract, on human colorectal adenocarcinoma HT29 cell, and rat colon specimens treated with lipopolysaccharide (LPS) to induce an inflammatory stimulus, a well established model of acute ulcerative colitis. In this context, the activities of different biomarkers of inflammation and lipid peroxidation such as ROS, myeloperoxidase (MPO), serotonin (5-HT), prostaglandin (PG)E-2, 8-iso-prostaglandin (8-iso-PG)F-2a, NF-kB, tumor necrosis factor (TNF)alpha and interleukin (IL)-6 were assessed. We found that chamomile extract was as effective as sulfasalazine (2 mg/ml) in reducing the production of MPO, 5-HT, IL-6, NF-kB, TNF alpha, PGE(2) and 8-iso-PGF(2 alpha), after inflammatory stimulus. The observed modulatory effects support a rationale use of chamomile supplementation as a promising pharmacological tool for the prevention and management of ulcerative colitis in humans. Copyright (C) 2016 John Wiley & Sons, Ltd.