CHARACTERIZATION OF 42 POLYMORPHIC MICROSATELLITE LOCI IN MIMULUS RINGENS (PHRYMACEAE) USING ILLUMINA SEQUENCING
CHARACTERIZATION OF 42 POLYMORPHIC MICROSATELLITE LOCI IN MIMULUS RINGENS (PHRYMACEAE) USING ILLUMINA SEQUENCING
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DOI：
10.3732/ajb.1200180
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发表时间：
2012-12-01
影响因子：
3
通讯作者：
Trapnell, Dorset W.
中科院分区：
文献类型：
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作者：
Nunziata, Schyler O.;Karron, Jeffrey D.;Trapnell, Dorset W.
研究前提：为研究湿地多年生草本植物Mimulus ringens （Phrymaceae）的交配模式和种群遗传结构，对其微卫星标记进行了分离和鉴定。方法与结果：从美国俄亥俄州一个单一种群的24个个体中共鉴定出42个多态性位点。每个位点的等位基因数为2 ~ 9个，观察到的杂合度中位数为0.435。结论：大量的多态性位点将使研究人员能够量化该物种大量自然种群的雄性适应性、多父性、自交和双亲本近交模式。这些标记也将允许对遗传结构的精细模式进行详细的研究。
Premise of the study: Microsatellite markers were isolated and characterized in Mimulus ringens (Phrymaceae), a herbaceous wetland perennial, to facilitate studies of mating patterns and population genetic structure.Methods and Results: A total of 42 polymorphic loci were identified from a sample of 24 individuals from a single population in Ohio, USA. The number of alleles per locus ranged from two to nine, and median observed heterozygosity was 0.435.Conclusions: This large number of polymorphic loci will enable researchers to quantify male fitness, patterns of multiple paternity, selfing, and biparental inbreeding in large natural populations of this species. These markers will also permit detailed study of fine-scale patterns of genetic structure.