LOCALIZATION AND SYNTHESIS OF HYALURONIC-ACID IN THE CUMULUS CELLS AND MURAL GRANULOSA-CELLS OF THE PREOVULATORY FOLLICLE
LOCALIZATION AND SYNTHESIS OF HYALURONIC-ACID IN THE CUMULUS CELLS AND MURAL GRANULOSA-CELLS OF THE PREOVULATORY FOLLICLE
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DOI：
10.1016/0012-1606(92)90192-j
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发表时间：
1992-06-01
影响因子：
2.7
通讯作者：
HASCALL, VC
中科院分区：
文献类型：
--
作者：
SALUSTRI, A;YANAGISHITA, M;HASCALL, VC
附壁和卵丘颗粒细胞合成透明质酸(HA)和膨胀素，对卵泡刺激素和成熟卵母细胞产生的可溶性因子(S)作出反应。在本研究中，我们检测了排卵前活体两种细胞群的HA合成和细胞外基质的组织。将人绒毛膜促性腺激素注射到孕马血清促性腺激素刺激的动物体内后，卵丘细胞-卵母细胞复合体(COC)和邻近胃窦的壁状颗粒细胞(胃窦颗粒细胞)的HA合成逐渐增加。壁层最外层的颗粒细胞(外周颗粒细胞)不合成HA。在体外，完全扩增后的COCs的HA净合成量为∼4pg/cell，而排卵期COCs的总HA含量和细胞数(∼11ngHA和∼3000cell/COC)约是体外扩增的COCs(∼4ngHA和∼1000cell/COC)的3倍。排卵后COCs细胞含量的增加可能主要是由于近侧壁层颗粒细胞对卵母细胞因子(S)的反应而合成HA，并结合到扩张的COC细胞外基质中的结果。以卵丘细胞团包裹的卵母细胞(完整的COCs)为条件的培养液，其HA刺激活性仅为同等数量的分离卵母细胞条件培养液的10-20%。此外，与无细胞预培养相比，卵丘细胞预培养5小时后，分离卵母细胞条件培养液的HA刺激活性显著降低(∼为70%)。结果表明，颗粒膜各亚区HA合成的差异取决于卵母细胞因子的扩散梯度(S)。
Mural and cumulus granulosa cells synthesize hyaluronic acid (HA) and expandin vitroin response to follicle-stimulating hormone and a soluble factor(s) produced by fully grown oocytes. In the present study we examined HA synthesis and extracellular matrix organization by the two cell populationsin vivoduring the preovulatory period. After injection of human chorionic gonadotropin into pregnant mares' serum gonadotropin-primed animals, a progressive increase in HA synthesis was observed by the cumulus cell-oocyte complex (COC), and by the mural granulosa cells adjacent to the antrum (antral granulosa cells). The outermost layers of mural granulosa cells (peripheral granulosa cells) did not synthesize HA. Net HA synthesis was ∼4 pg/cell for COCs isolated after full expansion induced eitherin vivoorin vitro, whereas the total HA content and cell number in the ovulated COC (∼11 ng HA and ∼3000 cells per COC) were about threefold higher than for COCs expandedin vitro(∼4 ng HA and ∼1000 cells per COC). The increased cell content of ovulated COCs appears to be primarily the result of inclusion of proximal mural granulosa cells which synthesize HA in response to the oocyte factor(s) and become incorporated in the expanded COC extracellular matrix mass. Media conditioned by oocytes enclosed in the cumulus cell mass (intact COCs) contained only 10–20% of the HA-stimulatory activity of media conditioned by an equal number of isolated oocytes when tested on mural granulosa cell cultures. Further, HA-stimulatory activity of media conditioned by isolated oocytes was dramatically reduced (∼70%) by preincubation for 5 hr with cumulus cells compared to preincubation in the absence of cells. The results suggest that differences in HA synthesis between subregions of membrana granulosa depend on a diffusion gradient of the oocyte factor(s).