Immunohistochemical evaluation of canine peripheral nerve sheath tumors and other soft tissue sarcomas
Immunohistochemical evaluation of canine peripheral nerve sheath tumors and other soft tissue sarcomas
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DOI：
10.1354/vp.41-4-307
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发表时间：
2004-07-01
影响因子：
2.4
通讯作者：
Tateyama, S
中科院分区：
文献类型：
--
作者：
Chijiwa, K;Uchida, K;Tateyama, S
检查了 17 例犬周围神经鞘瘤（PNST）、11 例恶性 PNST（MPNST）和 6 例良性 PNST（BPNST）。 6 只患有 BPNST 的狗中，有 5 只的预后良好，而所有患有 MPNST 的狗均在最后一次手术切除后 2 年内死亡。 1个BPNST形成具有MPNST特征的复发肿块。组织病理学上，MPNST的主要肿瘤细胞呈梭形或圆形，具有上皮样特征。其他非典型细胞具有丰富的颗粒状细胞质，或者是具有高碘酸希夫阳性细胞质球的多核巨细胞。此外，两个 MPNST 含有软骨和骨化生。相反，大多数 BPNST 表现出神经鞘瘤的典型特征。或神经纤维瘤，而两个 BPNST 具有非典型形态。一种 BPNST 包括上皮样细胞增殖和一些显示核异型性的肿瘤细胞。免疫组织化学显示，MPNST 中常见波形蛋白 (100%)、S-100 (73%)、神经生长因子受体 (NGFR，64%) 和肌红蛋白 (64%) 的表达。两个具有非典型组织学表现的 BPNST 呈波形蛋白、S-100、NGFR 和神经元特异性烯醇化酶阳性，其中之一对细胞角蛋白具有中等免疫反应性。大多数 BPNST 的神经胶质原纤维酸性蛋白以及 S-100 和 NGFR 呈阳性。尽管大多数横纹肌肉瘤 (RMS) 和犬血管外皮细胞瘤 (CHP) 也表现出 S-100 局灶性免疫反应性，但大多数 RMS 肌红蛋白呈强阳性，而 NGFR 呈阴性。大多数 CHP (80%) 表现出局灶性 α-平滑肌肌动蛋白 (α-SMA) 表达，而所有 PNST 均为阴性。这些结果表明 NGFR 和 α-SMA 的免疫组织化学可能有助于分别区分犬 PNST 与 RMS 或 CHP。
Seventeen cases of canine peripheral nerve sheath tumors (PNSTs), 11 malignant PNSTs (MPNSTs), and six benign PNSTs (BPNSTs) were examined. The prognosis in five of six dogs with BPNSTs was excellent, whereas all dogs with MPNSTs died within 2 years after the last surgical resection. One BPNST formed a recurrent mass with features of a MPNST Histopathologically, the predominant tumor cell of MPNSTs was either spindle or round in shape with epithelioid characteristics. Other atypical cells had abundant granular cytoplasm or were multinucleated giant cells with periodic acid-Schiff-positive cytoplasmic globules. Furthermore, two MPNSTs contained cartilaginous and osseous metaplasia. On the contrary, most BPNSTs exhibited typical features of schwannoma. or neurofibroma, whereas two BPNSTs had atypical morphology. One BPNST consisted of epithelioid cell proliferation with some tumor cells revealing nuclear atypia. Immunohistochemically, the expression of vimentin (100%), S-100 (73%), nerve growth factor receptor (NGFR, 64%), and myoglobin (64%) was commonly found in MPNSTs. The two BPNSTs with atypical histologic appearances were positive for vimentin, S-100, NGFR, and neuron-specific enolase, and one of these had moderate immunoreactivity for cytokeratin. Most BPNSTs were positive for glial fibrillary acidic protein, as well as S-100 and NGFR. Although most rhabdomyosarcomas (RMSs) and canine hemangiopericytomas (CHPs) also showed focal immunoreactivity for S-100, most RMSs were intensely positive for myoglobin and negative for NGFR. Most CHPs (80%) exhibited focal alpha-smooth muscle actin (alpha-SMA) expression, whereas all PNSTs were negative. These results indicate that immunohistochemistry for NGFR and alpha-SMA might be useful for differentiating canine PNSTs from RMSs or CHPs, respectively.