A microfluidic platform with permeable walls for the analysis of vascular and extravascular mass transport
A microfluidic platform with permeable walls for the analysis of vascular and extravascular mass transport
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DOI：
10.1007/s10404-016-1775-5
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发表时间：
2016-08-01
影响因子：
2.8
通讯作者：
Decuzzi, P.
中科院分区：
文献类型：
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作者：
Manneschi, C.;Pereira, R. C.;Decuzzi, P.
其中葡聚糖的分子量和珠粒的直径对于40和250 kDa葡聚糖为约6 × 10（-5）至2 × 10（-5）cm/s，对于1.5 μ m珠粒为最大为0。所提供的数据表明，所提出的微流控芯片的潜力，用于分析血管和血管外的质量运输，在多个空间和时间尺度上，在各种疾病，涉及跨血管壁的差异渗透。血池和血管外基质之间的界面在生理和病理条件下调节分子、纳米颗粒和细胞的转运中是基本的。在这项工作中，提出了一种微流控芯片，包括两个平行的微通道连接横向通过阵列的高纵横比微柱，构成的可渗透的血管膜。一个双步骤的光刻工艺结合复制品成型的方法，用于实现80个不同的阵列的微柱，表现出三个横截面几何形状（矩形，椭圆形和弯曲）;两个方向（正常和平行）相对于流动;和各种宽度和间隙尺寸，分别从10至20 μ m和2至5 μ m。与常规矩形结构相比，弯曲柱提供更高的抗弯刚度、更低的粘合剂相互作用和更小的通道内分离距离。具体来说，10亩宽的弯曲支柱，铺设平行于流动，提供了最高的机械稳定性。为了评估血管渗透性，用透明质酸水凝胶填充血管外通道，同时将荧光葡聚糖分子和校准的聚苯乙烯珠注射到血管通道中。观察到膜渗透性随着葡聚糖的分子量和珠粒的直径而降低，对于40和250 kDa葡聚糖，范围为约6 × 10（-5）至2 × 10（-5）cm/s，对于1.5 μ m珠粒，范围高达0。所提供的数据表明，所提出的微流控芯片的潜力，用于分析血管和血管外的质量运输，在多个空间和时间尺度上，在各种疾病，涉及跨血管壁的差异渗透。
with the molecular weight of Dextran and diameter of the beads, ranging from about 6 x 10(-5) to 2 x 10(-5) cm/s for 40 and 250 kDa Dextran and up to zero for 1.5 mu m beads. The presented data demonstrate the potential of the proposed microfluidic chip for analyzing the vascular and extravascular mass transport, over multiple spatial and temporal scales, in a variety of diseases involving differential permeation across vascular walls. The interface between the blood pool and the extravascular matrix is fundamental in regulating the transport of molecules, nanoparticles and cells under physiological and pathological conditions. In this work, a microfluidic chip is presented comprising two parallel microchannels connected laterally via an array of high aspect ratio micropillars, constituting the permeable vascular membrane. A double-step lithographic process combined with a replica molding approach is employed to realize 80 different arrays of micropillars exhibiting three cross-sectional geometries (rectangular, elliptical and curved); two orientations (normal and parallel) with respect to the flow; and a variety of width and gap sizes, respectively, ranging from 10 to 20 mu m and 2 to 5 mu m. As compared to conventional rectangular structures, the curved pillars provide higher bending stiffness, lower adhesive interactions, and smaller intrachannel separation distances. Specifically, 10-mu m-wide curved pillars, laying parallel to the flow, offered the highest mechanical stability. To assess vascular permeability, the extravascular channel was filled with a hyaluronic acid hydrogel, while fluorescent Dextran molecules and calibrated polystyrene beads were injected in the vascular channel. Membrane permeability was observed to reduce with the molecular weight of Dextran and diameter of the beads, ranging from about 6 x 10(-5) to 2 x 10(-5) cm/s for 40 and 250 kDa Dextran and up to zero for 1.5 mu m beads. The presented data demonstrate the potential of the proposed microfluidic chip for analyzing the vascular and extravascular mass transport, over multiple spatial and temporal scales, in a variety of diseases involving differential permeation across vascular walls.