SIMULTANEOUS MEASUREMENTS OF HEPATIC AND PORTAL VENOUS BLOOD FLOW IN SHEEP AND DOG
SIMULTANEOUS MEASUREMENTS OF HEPATIC AND PORTAL VENOUS BLOOD FLOW IN SHEEP AND DOG
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DOI：
10.1152/ajplegacy.1969.216.4.946
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发表时间：
1969-01-01
影响因子：
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通讯作者：
BERGMAN, EN
中科院分区：
文献类型：
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作者：
KATZ, ML;BERGMAN, EN
方法动物、饮食和手术/静脉分离。羊和狗都被使用了，而且都完全习惯了搬运。每只动物都被关在单独的实验室围栏里，室温保持在65华氏度到70华氏度之间。绵羊被剪毛，成熟，杂交母羊，要么没有怀孕，要么是双胞胎怀孕。双胎妊娠绵羊是通过腹部X线片的方法选择的，在分娩后36天内。饮食由自由喂养的紫花苜蓿干草组成。然而，每天早上和晚上都要喂新鲜的干草，大部分饲料消耗都发生在这两个时间段。除特殊饲喂实验外，所有实验均在早晨饲喂后3小时和禁食3天后进行。任何时候都可以喝水和舔盐。在所有绵羊身上，如前所述，通过外科手术将聚乙烯管植入股动脉、肝静脉、门静脉、肠系膜静脉和股静脉。这些动物制剂的有效时间各不相同，但许多在手术后几个月内仍然有效。这些动物从未被用于实验测量，直到它们正常进食，至少在手术后5-7天。在狗身上，实验要么在Nembutal麻醉下的急性制剂中进行，要么在长期准备的动物吸收后状态下进行。这些狗是根据Shoemaker和同事的方法进行手术准备的(29)，长期准备的动物直到它们从手术的压力中完全恢复才被使用。血瘀法的理论方面。Roe及其同事的门脉血流方法(23)包括给予预充剂，然后将PAH持续注入肠系膜静脉，在一段时间的平衡后，这允许门静脉血液持续稀释注入的PAH。该方法(23)最初测量门脉血浆流量，但我们对该方法进行了修改，以便直接测量全血流量。这种使用全血而不是血浆进行分析，从而消除了由于测量压缩的细胞体积而引起的任何误差，也消除了可能由于多环芳烃扩散到红细胞中而引起的任何误差。因此，进入门静脉系统的多环芳烃的量等于通过门静脉血液离开的多环芳烃的量，因此：
METHODSAnimals, diet, and surgicul/veparation. Both sheep and dogs were used and all were thoroughly accustomed to handling. Each animal was housed in an individual laboratory pen and the room temperature was kept between 65 and 70 F. The sheep were sheared, mature, cross-bred ewes and were either nonpregnant or twinpregnant. The twin-pregnant sheep were selected bv means of abdominal radiography and were within 36 days of parturition. The diet consisted of an alfalfa-grass hay fed ad libitum. Fresh hay was given each morning and evening, however, and most feed consumption occurred at these times. Except for specific feeding experiments, all experiments were performed 3 hr after the morning feeding and also after 3 days of fasting. Water and a salt lick were available at all times. In all sheep, polyvinyl cannulas were surgically implanted into a femoral artery and the hepatic, portal, mesenteric, and femoral veins, as previously described (20). These animal preparations had variable periods of usefulness, but many remained patent for several months following the surgery. The animals were never used for experimental measurements until they were eating normally and at least 5-7 days after surgery. In dogs, the experiments were performed either in the acute preparation under Nembutal anesthesia or in chronically prepared animals in the postabsorptive state. The dogs were surgically prepared according to the method of Shoemaker and co-workers(29) and the chronically prepared animals were not used until they had fully recovered from the stress of surgery. Theoretical aspects of the blood jlow method. The portal blood flow method of Roe and associates (23) consisted of giving a priming dose followed by a constant infusion of PAH into a mesenteric vein and, after a period of time for equilibration, this allowed a constant dilution of the infused PAH by the portal vein blood. The method(23) originally measured portal plasma flow, but we have modified the method so that whole blood flow would be measured directly. This use of whole blood for analysis, rather than plasma, thus eliminates any errors due to the measurement of the packed cell volume and also eliminates any errors which might result from a diffusion of PAH into the red blood cells. Thus, the amount of PAH entering the portal system equals the amount of PAH leaving by way of the portal blood and therefore: