Interaction between 17β-estradiol degradation and nitrification in mariculture wastewater by Nitrosomonas europaea and MBBR
Interaction between 17β-estradiol degradation and nitrification in mariculture wastewater by Nitrosomonas europaea and MBBR
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欧洲亚硝化单胞菌和 MBBR 海水养殖废水中 17β-雌二醇降解和硝化作用的相互作用
DOI：
10.1016/j.scitotenv.2019.135846
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发表时间：
2020-02-25
影响因子：
9.8
通讯作者：
Liu, Dezhao
中科院分区：
文献类型：
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作者：
Li, Changwei;Lan, Lihua;Liu, Dezhao
采用氨氧化细菌NitroSomonas uropaea和移动床生物膜反应器(MBBR)研究了人工养殖废水中17β-雌二醇(E_2)的降解与硝化的关系。间歇实验表明，在氨存在下，欧洲拟青霉对废水中E2的降解符合零级反应动力学(r(2)=0.944，4.07mg/L h(-1))。1 mg/L的雌二醇可使接种的氮藻的亚硝酸盐产量下降77.8%。当E_2浓度从50 ng/L增加到1 mg/L时，对氨氧化的抑制作用增强。值得注意的是，当E_2浓度降至50 ng/L时，对N-硝酸盐生成、细菌密度和氨单加氧酶活性仍有显著的干扰作用。尽管如此，后续68天的连续降解试验表明，基准MBBR可以去除84.5%-98.7%的E2。而当进水E_2从10µg/L增加到1 mg/L时，氨氮的去除从94.7%+/-2.1%显著下降到85.6%+/-2.1%(p<；0.05)，同时E_2的去除也得到了提高(从84.5%+/-2.0%降到98.7%+/-0.4%，p&t；0.05)，说明了异养生物在E_2降解中的重要作用。相反，亚硝酸盐氧化不受E2暴露的影响，与E2浓度无关。总而言之，硝化作用对去除E2是有效的，而E2干扰氨氧化过程，但这种干扰在反应器水平上可以忽略不计，因为在实际田间条件下，E2水平很低。(C)2019爱思唯尔B.V.保留所有权利。
This study investigated the relation between 17 beta-estradiol (E2) degradation and nitrification in synthetic mariculture wastewater by ammonia oxidizing bacterium Nitrosomonas europaea and moving bed biofilm reactor (MBBR). Batch experiments showed that E2 degradation by N. europaea in wastewater followed zero-order reaction kinetics (r(2) = 0.944, 4.07 mu g/ L h(-1)) when ammonia presented. Nitrite yield in N. europaea inoculation decreased by 77.8% exposed to 1 mg/L E2. The inhibitory impact on ammonia oxidation was enhanced with increasing E2 dosage from 50 ng/L to 1 mg/L. Notably, E2 as low as 50 ng/L still had significant interference with nitrite production, bacterial density and ammonia monooxygenase (AMO) activity of N. europaea. Still, the following continuous 68-day degradation test revealed that 84.5%-98.7% E2 could be removed by a benchscale MBBR. Whereas, ammonia removal remarkably decreased from 94.7% +/- 2.1% to 85.6% +/- 2.1% (p < .05) along with the enhanced E2 removal (from 84.5% +/- 2.0% to 98.7% +/- 0.4%, p < .05) when inlet E2 increased from 10 mu g/L to 1 mg/L, indicating the great role of heterotrophs in E2 degradation. In contrast, nitrite oxidation was not affected upon E2 exposure irrespective of E2 concentrations. In summary, nitrification was effective in removing E2, while E2 interfered with ammoxidation process, but this interference was negligible at the reactor level given the low level of E2 in practical field conditions. (C) 2019 Elsevier B.V. All rights reserved.