Regulation of gene expression in lectin-stimulated or lymphokine-stimulated T lymphocytes. Effects of cyclosporine.
Regulation of gene expression in lectin-stimulated or lymphokine-stimulated T lymphocytes. Effects of cyclosporine.
复制标题
凝集素刺激或淋巴因子刺激的 T 淋巴细胞中基因表达的调节。
DOI：
10.1097/00007890-198808001-00016
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发表时间：
1988
期刊：
影响因子：
6.2
通讯作者：
Nowell,PC
中科院分区：
文献类型：
--
作者：
Reed,JC;Prystowsky,MB;Nowell,PC
检查了环孢菌素对促有丝分裂凝集素和白细胞介素 2 (IL-2) 在 T 淋巴细胞中诱导的基因表达的影响。在抑制人外周血淋巴细胞增殖[几乎等于]90%的浓度下使用，CsA不同程度地抑制植物血凝素刺激的各种基因的表达，其中IL-2的mRNA水平被抑制[几乎等于]100%，c-myc和N-ras被抑制[几乎等于]80%，c-fos和IL-2受体被抑制[几乎等于]80%。 50%。比较 CsA 对用伴刀豆球蛋白 A 或 IL-2 刺激的克隆鼠 T 细胞 (L2) 中基因表达的作用，结果表明，当 Con A 诱导时，CsA 特异性阻断 c-myc 和 p53 原癌基因 mRNA 的积累，但当 IL-2 诱导时则不会。总而言之，这些发现表明多种途径可以控制特定基因的表达，并表明 CsA 仅干扰 T 淋巴细胞中基因表达的部分调节途径。
The effects of cyclosporine were examined on gene expression induced in T lymphocytes by mitogenic lectins and interleukin 2 (IL-2). Used at concentrations that inhibited proliferation of human peripheral blood lymphocytes by [almost equal to] 90%, CsA suppressed, to different extents, the phytohemagglutinin-stimulated expression of various genes, with levels of mRNAs for IL-2 being inhibited by [almost equal to] 100%, c-myc and N-ras by [almost equal to] 80%, and c-fos and IL-2 receptors by [almost equal to] 50%. Comparisons of the actions of CsA on gene expression in a cloned murine T cell (L2), stimulated with concanavalin A or IL-2, demonstrated that CsA specifically blocked the accumulation of mRNAs for the c-myc and p53 protooncogenes when induced by Con A, but not when induced by IL-2. Taken together, these findings indicate that several pathways can control the expression of a particular gene, and suggest that CsA interferes with only some of these regulatory pathways of gene expression in T lymphocytes.