Th1/Th2 profile in peripheral blood in atopic cough and atopic asthma
Th1/Th2 profile in peripheral blood in atopic cough and atopic asthma
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DOI：
10.1046/j.1365-2222.2003.01578.x
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发表时间：
2003-01-01
影响因子：
6.1
通讯作者：
Nakamura, H
中科院分区：
文献类型：
--
作者：
Shirai, T;Suzuki, K;Nakamura, H
背景嗜酸性气管支气管炎伴咳嗽超敏性，简称特应性咳嗽，是慢性咳嗽的重要病因。不存在气道高反应性的原因尚不清楚，与哮喘不同，哮喘是一种Th2细胞因子介导的疾病。目的比较特应性咳嗽和特应性哮喘患者外周血中1型辅助性T细胞(Th1)/Th2的平衡，在单细胞水平上评估细胞内细胞因子的产生。方法选取36例患者，其中特应性咳嗽10例，特应性哮喘18例，对照组8例。流式细胞术检测CD4(+) T细胞的细胞内IL-4和ifn - γ。结果特应性咳嗽和哮喘患者经12-肉豆酸酯佛波/离子霉素刺激后产生ifn - γ -/ il -4的CD4(+) T细胞比例明显低于正常人。在特应性咳嗽患者中，ifn - γ -/ il -4生成细胞的比例明显高于特应性哮喘患者。而异位性哮喘患者产生il -4的CD4(+) T细胞比例明显高于正常对照，异位性咳嗽患者与正常对照无显著差异。受试者之间产生ifn - γ细胞的比例无显著差异。总体而言，总IgE水平与产生il -4的细胞呈正相关，与产生ifn - γ -/ il -4的细胞比例呈负相关。结论Th2细胞因子在特应性咳嗽中的优势程度低于特应性哮喘，提示Th1/Th2平衡与特应性状态的关系。
Background Eosinophilic tracheobronchitis with cough hypersensitivity, abbreviated as atopic cough, is an important cause of chronic cough. The reason for the absence of airway hyper-responsiveness is unknown, differing from asthma, a Th2 cytokine-mediated disorder.Objective To compare the type 1 helper T cell (Th1)/Th2 balance in the peripheral blood from subjects with atopic cough and atopic asthma, we assessed the intracellular cytokine production at the single-cell level.Methods Thirty-six subjects (10 patients with atopic cough, 18 with atopic asthma, and eight control subjects) were included. Intracellular IL-4 and IFN-gamma were detected in CD4(+) T cells by flow cytometry.Results A significantly lower ratio of IFN-gamma-/IL-4-producing CD4(+) T cells after phorbol 12-myristate acetate/ionomycin stimulation was found in patients with atopic cough and atopic asthma compared with normal subjects. In comparison between atopic patients, the ratio of IFN-gamma-/IL-4-producing cells was significantly higher in atopic cough than in atopic asthma. However, the proportion of IL-4-producing CD4(+) T cells was significantly higher in patients with atopic asthma than in normal control subjects and no significant difference was detected between patients with atopic cough and normal subjects. No significant difference in the proportion of IFN-gamma-producing cells was found between the subjects. Overall, the total IgE levels were positively correlated to the IL-4-producing cells and inversely correlated to the ratio of IFN-gamma-/IL-4-producing cells.Conclusion These results show the lower degree of Th2 cytokine predominance in atopic cough compared with atopic asthma and suggest the relation between the Th1/Th2 balance and atopic status.