Infection of cultured central nervous system neurons with a defective herpes simplex virus 1 vector results in stable expression of Escherichia coli beta-galactosidase.
Infection of cultured central nervous system neurons with a defective herpes simplex virus 1 vector results in stable expression of Escherichia coli beta-galactosidase.
复制标题
用有缺陷的单纯疱疹病毒 1 载体感染培养的中枢神经系统神经元会导致大肠杆菌 β-半乳糖苷酶的稳定表达。
DOI：
10.1073/pnas.87.3.1149
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发表时间：
1990
影响因子：
11.1
通讯作者：
Freese,A
中科院分区：
文献类型：
--
作者：
Geller,AI;Freese,A
我们已经开发了一个有缺陷的单纯疱疹病毒（HSV）载体系统，允许引入几乎任何基因到哺乳动物中枢神经系统神经元。原型载体pHSVlac含有将大肠杆菌lacZ基因置于HSV-1立即早期4/5启动子控制下的转录单位。使用HSV-1温度敏感突变体ts K作为辅助病毒来繁殖pHSVlac。感染原代培养的大鼠神经元，这些神经元来源于整个中枢神经系统的各个区域，包括脊髓、小脑、丘脑、基底神经节、海马、枕叶皮质、颞叶皮质和额叶皮质，导致高水平β-半乳糖苷酶的稳定表达至少2周，而没有细胞损伤。由于其他基因可以从pHSVlac表达，HSV-1载体可能被证明可用于将基因递送到中枢神经系统神经元中，用于神经系统生理学的研究或用于对神经病症进行基因治疗。
We have developed a defective herpes simplex virus (HSV) vector system that permits the introduction of virtually any gene into mammalian central nervous system neurons. The prototype vector, pHSVlac, contains a transcription unit that places the Escherichia coli lacZ gene under the control of the HSV-1 immediate early 4/5 promoter. pHSVlac was propagated using the HSV-1 temperature-sensitive mutant ts K as helper virus. Infection of rat neurons in primary culture derived from various regions throughout the central nervous system, including spinal cord, cerebellum, thalamus, basal ganglia, hippocampus, occipital cortex, temporal cortex, and frontal cortex, resulted in stable expression of high levels of beta-galactosidase for at least 2 weeks, without cell damage. Since other genes can be expressed from pHSVlac, HSV-1 vectors may prove useful for delivery of genes into central nervous system neurons for studies on nervous system physiology or to perform gene therapy for neurological conditions.