Denitrobacterium detoxificans gen. nov., sp nov., a ruminal bacterium that respires on nitrocompounds
Denitrobacterium detoxificans gen. nov., sp nov., a ruminal bacterium that respires on nitrocompounds
复制标题
DOI：
10.1099/00207713-50-2-633
复制
发表时间：
2000-03-01
影响因子：
2.8
通讯作者：
Allison, MJ
中科院分区：
文献类型：
--
作者：
Anderson, RC;Rasmussen, MA;Allison, MJ
从牛瘤胃中分离到一组新的革兰氏阳性、高G+C （56 ~ 60 mol%）的厌氧细菌，4株菌株均不运动，不产生孢子。该菌株不产生吲哚或H2S，也不水解明胶。每个菌株的细胞形态相似，呈棒状（0.5-1.0 × 1.0-1.5 μ m），尽管有时存在球茎端。这四种菌株都不能通过氧化各种潜在的可发酵底物来生长，而是通过厌氧呼吸过程，氧化氢，甲酸盐或乳酸来还原各种氧化氮化合物来获得生长所需的能量。氧化三甲胺和二甲基亚砜也被用作电子受体。4株菌株的16S rRNA基因序列同源性均大于99%。NPOH1(T)、NPOH2、NPOH3和MAJ1这四种菌株的16S rRNA基因序列与GenBank中可用的序列最匹配的是放线菌纲中表型不同的厌氧菌——肾球Coriobacterium glomerans（86%序列相似性）。为了适应这些细菌，研究人员提出了一个新的属和种——脱硝杆菌（Denitrobacterium decificans），将其安置在科中。型菌株NPOH1(T) （ATCC 700546(T)）在测试的孵育温度范围（32-39℃）内同样生长良好。
A new group of anaerobic, Gram-positive, high G+C (56-60 mol%) bacteria was isolated from the bovine rumen, Of four strains characterized, all were nonmotile and none produced spores. The isolates did not produce indole or H2S and did not hydrolyse gelatin. Cells of each strain exhibited similar rod-shaped morphology (0.5-1.0 x 1.0-1.5 mu m) although bulbous ends were sometimes present. None of the four strains were able to grow via oxidation of a variety of potentially fermentable substrates but rather obtained energy for growth via anaerobic respiration processes, oxidizing hydrogen, formate or lactate for reduction of various oxidized nitrogen compounds. Trimethylamine oxide and DMSO were also used as electron acceptor. All four strains shared greater than 99% 16S rRNA gene sequence identity. The closest match found between the 16S rRNA gene sequence of all four strains, NPOH1(T), NPOH2, NPOH3 and MAJ1, to sequences available in GenBank was that of Coriobacterium glomerans (86 % sequence similarity), a phenotypically dissimilar anaerobe within the class Actinobacteria, To accommodate these bacteria the creation of a new genus and species, Denitrobacterium detoxificans, for placement within the family Coriobacteriaceae is proposed. The type strain, NPOH1(T) (ATCC 700546(T)), grew equally well over a narrow range of incubation temperatures tested (32-39 degrees C).