Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival
Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival
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DOI：
10.1158/0008-5472.can-04-0637
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发表时间：
2004-06-01
期刊：
影响因子：
11.2
通讯作者：
Takahashi, T
中科院分区：
文献类型：
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作者：
Takamizawa, J;Konishi, H;Takahashi, T
在这项研究中，我们首次报告了let-7 microRNA在人类肺癌中的表达减少。有趣的是，143例肺癌病例，经历了潜在的根治性切除术可以分为两个主要组，根据let-7表达在无监督的分层分析，显示潜在的根治性切除术后的生存率显着较短的情况下，降低let-7表达（P = 0.0003）。多变量考克斯回归分析显示，这种预后影响与疾病分期无关（风险比= 2.17; P = 0.009）。此外，let-7在A549肺腺癌细胞系中的过表达在体外抑制肺癌细胞的生长。这项研究代表了let-7表达减少以及这种microRNA改变的潜在临床和生物学效应的首次报道。
In this study, we report for the first time reduced expression of the let-7 microRNA in human lung cancers. Interestingly, 143 lung cancer cases that had undergone potentially curative resection could be classified into two major groups according to let-7 expression in unsupervised hierarchical analysis, showing significantly shorter survival after potentially curative resection in cases with reduced let-7 expression (P = 0.0003). Multivariate COX regression analysis showed this prognostic impact to be independent of disease stage (hazard ratio = 2.17; P = 0.009). In addition, overexpression of let-7 in A549 lung adenocarcinoma cell line inhibited lung cancer cell growth in vitro. This study represents the first report of reduced expression of let-7 and the potential clinical and biological effects of such a microRNA alteration.