Formation characteristics of an anoxygenic photosynthetic bacterial biofilm in a photorotating biological contactor for azo dye wastewater treatment
Formation characteristics of an anoxygenic photosynthetic bacterial biofilm in a photorotating biological contactor for azo dye wastewater treatment
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DOI：
10.1002/jctb.4303
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发表时间：
2015
影响因子：
3.4
通讯作者：
Xingzu Wang;Yu Wang;Xiang Cheng;Dezhi Sun;Yiwei Ren;Guihua Xu
中科院分区：
文献类型：
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作者：
Xingzu Wang;Yu Wang;Xiang Cheng;Dezhi Sun;Yiwei Ren;Guihua Xu
背景：由于厌氧光合细菌（APB）生物膜形成能力差，大多数用于废水处理的光生物反应器都是基于悬浮培养物。本研究采用光旋转生物接触器（PRBC）对含偶氮废水的APB生物膜进行培养，重点研究了APB生物膜的形成及其与其它微生物和胞外多糖（EPS）的关系。结果PRBC成功地用于APB生物膜的培养及化学需氧量和色度的去除。原位分析表明，APB生物膜的形成可分为两个不同的阶段：发育和分化。在发育阶段，非光合丝状微生物最初在圆盘表面增殖并促进APB的粘附。在分化阶段，丝状微生物及其EPS基质将APB笼在内层，稳定了APB生物膜。在生物膜分层过程中，生物膜生物量和APB数量分别达到24.2 ± 0.63 g L−1和4.8 ± 0.6 × 108 CFU（g干生物膜）−1。成熟生物膜中占优势的APB被鉴定为Rhodopropiculium、Rhodomicrobium和Chlorobium。结论在非光合微生物的辅助下，生物膜形成能力较差的APB在PRBC中形成了稳定的生物膜。© 2014化学工业协会
BACKGROUND Most photobioreactors used for wastewater treatment with anoxygenic photosynthetic bacteria (APB) are based on suspended cultures because APB have poor biofilm-forming ability. In this study, a photo-rotating biological contactor (PRBC) was applied for the culture of APB biofilm using azo wastewater, with a particular emphasis on the formation of APB biofilm and its relationship to other microorganisms and exopolysaccharides (EPS). RESULTS The PRBC was successfully used for the cultivation of APB biofilm and in the removal of chemical oxygen demand and color. In situ analysis showed that APB biofilm formation can be divided into two distinct stages: development and differentiation. In the development stage, nonphotosynthetic filamentous microorganisms initially proliferated on a disc surface and promoted the adherence of APB. In the differentiation stage, filamentous microorganisms and their EPS matrices caged APB in the inner layer and stabilized the APB biofilm. During biofilm stratification, the biofilm biomass and APB numbers reached 24.2 ± 0.63 g L−1 and 4.8 ± 0.6 × 108 CFU (g dry biofilm)−1, respectively. The dominant APB in the mature biofilm were identified as Rhodopseudomonas, Rhodomicrobium, and Chlorobium. CONCLUSION APB with poor biofilm-forming ability formed a stable biofilm in the PRBC with the aid of nonphotosynthetic microorganisms. © 2014 Society of Chemical Industry