Formaldehyde quantification using ampicillin is not selective
Formaldehyde quantification using ampicillin is not selective
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DOI：
10.1038/s41598-019-54610-3
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发表时间：
2019-12-04
影响因子：
4.6
通讯作者：
Hopkinson, Richard J.
中科院分区：
文献类型：
--
作者：
Reinbold, Raphael;John, Tobias;Hopkinson, Richard J.
甲醛(HCHO)是一种简单的、高活性的人体代谢物，但其生物化学性质尚不清楚。六六六研究中的一个限制因素是缺乏与生物样品相关的有效的六六六量化方法。我们描述了一种报道的基于荧光的HCHO检测方法的光谱研究，该方法涉及其与氨苄西林的反应。结果验证了甲醛-氨苄西林反应产物的结构和荧光性质。然而，氨苄西林与乙醛酸盐反应后，也观察到相同的加合物。相关的荧光团与其他生物相关的羰基化合物形成。总体而言，我们的研究表明，氨苄西林方法不能用于生物样品中甲醛的选择性检测和定量。
Formaldehyde (HCHO) is a simple and highly reactive human metabolite but its biochemistry is poorly defined. A limiting factor in HCHO research is lack of validated quantification methods for HCHO relevant to biological samples. We describe spectroscopic studies on a reported fluorescence-based HCHO detection method involving its reaction with ampicillin. The results validate the structure and fluorescence properties of the HCHO-ampicillin reaction product. However, the same adduct is observed after reaction of ampicillin with glyoxylate. Related fluorophores were formed with other biologically relevant carbonyl compounds. Overall, our studies suggest the ampicillin method is not reliable for selective detection and quantification of HCHO in biological samples.