A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis
A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis
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DOI：
10.1016/j.femsle.2004.11.012
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发表时间：
2005-01-15
影响因子：
2.1
通讯作者：
Shimamoto, T
中科院分区：
文献类型：
--
作者：
Ahmed, AM;Shinoda, S;Shimamoto, T
河流弧菌H-08942株分离自印度一名患有霍乱样腹泻的6个月大婴儿。该菌株显示出典型的SXT元件的多药耐药表型。对磺胺甲恶唑（Su）、甲氧苄啶（Tm）、氯霉素（Cm）、链霉素（Sm）耐药，对氨苄西林（Am）、呋喃唑酮（Fz）、萘啶酸（Na）、庆大霉素（Gm）耐药。SXT元件是霍乱弧菌衍生的整合和接合元件（ICE），其也被称为接合转座子。我们的目标是找到这些耐药表型和这种独特菌株中SXT元件存在之间的关系。利用PCR技术检测了SXT的抗生素抗性基因、整合酶基因和attP连接位点。克隆和测序结果表明，河流弧菌的SXT整合酶基因及其attP位点与霍乱弧菌的SXT整合酶基因及其attP位点相似，但不完全相同。河流弧菌SXT整合酶基因与霍乱弧菌SXT整合酶基因的同源性为99%，且河流弧菌SXT整合酶的attP位点（641 bp）较霍乱弧菌（785 bp）短且变异较大。河流弧菌的SXT有可能通过接合转移到大肠杆菌的实验室菌株上。在这里，我们报告的变异SXT元件的检测，在其他物种比霍乱弧菌，其整合酶基因和attP位点的分子特征和分析。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
Vibrio fluvialis strain H-08942 was isolated from an infant aged 6 months who was suffering from cholera-like diarrhea in India. This strain showed the typical multidrug-resistance phenotype of an SXT element. It was resistant to sulfamethoxazole (Su), trimethoprim (Tm), chloramphenicol (Cm) and streptomycin (Sm), in addition to other antibiotics such as ampicillin (Am), furazolidone (Fz), nalidixic acid (Na), and gentamicin (Gm). The SXT element is a Vibrio cholerae-derived integrative and conjugative element (ICE) that has also been referred to as a conjugative transposon. Our goal was to find a relationship between these resistant phenotypes and the presence of the SXT element in this unique strain. By using PCR, we detected the antibiotic resistance genes, the integrase gene and the attP attachment site of SXT element. Cloning and DNA sequencing results showed that both the SXT integrase gene and its attP site of V fluvialis were similar but not identical to those of V cholerae. The SXT integrase gene of V fluvialis has a 99% identity to that of V cholerae, and the attP site of SXT of V fluvialis is variant and shorter (641 bp) than that of V. cholerae (785 bp). It was possible for the SXT of V. fluvialis to be transferred by conjugation to a laboratory strain of Escherichia coli. Here, we report the detection of a variant SXT element in species other than V. cholerae, with molecular characterization and analysis of its integrase gene and its attP site. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.