METABOLIC EFFECTS AND CYCLIC-AMP LEVELS PRODUCED BY GLUCAGON, (1-N-ALPHA-TRINITROPHENYLHISTIDINE,12-HOMOARGININE)GLUCAGON AND FORSKOLIN IN ISOLATED RAT HEPATOCYTES
METABOLIC EFFECTS AND CYCLIC-AMP LEVELS PRODUCED BY GLUCAGON, (1-N-ALPHA-TRINITROPHENYLHISTIDINE,12-HOMOARGININE)GLUCAGON AND FORSKOLIN IN ISOLATED RAT HEPATOCYTES
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DOI：
10.1016/0167-4889(84)90071-5
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
GARCIASAINZ, JA
中科院分区：
文献类型：
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作者：
CORVERA, S;HUERTABAHENA, J;GARCIASAINZ, JA
[1-N α-三硝基苯组氨酸，12-高精氨酸]胰高血糖素（THG）是胰高血糖素对肝膜腺苷酸环化酶作用的有效拮抗剂。在离体肝细胞中，这种胰高血糖素类似物是cAMP蓄积的极弱部分激动剂，并且它阻断胰高血糖素产生的cAMP蓄积刺激。THG是一种完全激动剂，刺激大鼠肝细胞中的糖原分解、糖原生成和尿素合成，并且在大多数检查条件下不拮抗胰高血糖素的代谢作用。毛喉素增强刺激cAMP积累产生的胰高血糖素或THG，但不增强他们的代谢行动。毛喉素比胰高血糖素或THG刺激肝细胞代谢所需的cAMP水平增加似乎要大得多。这可能表明大鼠肝细胞中存在cAMP的功能区室化。cAMP介导的激素作用和参与的因素，除了cAMP，在介导的THG和胰高血糖素的影响可能存在的车厢建议。
[1-N.alpha.-Trinitrophenylhistidine,12-homoarginine]glucagon (THG) is a potent antagonist of the effects of glucagon on liver membrane adenylate cyclase. In isolated hepatocytes, this glucagon analog was an extremely weak partial agonist for cAMP accumulation, and it blocked the stimulation of cAMP accumulation produced by glucagon. THG was a full agonist for the stimulation of glycogenolysis, gluconeogenesis and urea synthesis in rat hepatocytes, and did not antagonize the metabolic effects of glucagon under most of the conditions examined. Forskolin potentiated the stimulation of cAMP accumulation produced by glucagon or THG, but did not potentiate their metabolic actions. A much larger increase in cAMP levels seemed to be required for the stimulation of hepatocyte metabolism by forskolin than by glucagon or THG. This may suggest the existence of a functional compartmentation of cAMP in rat hepatocytes. The possible existence of compartments in cAMP-mediated hormone actions and the involvement of factors, besides cAMP, in mediating the effects of THG and glucagon is suggested.