HIGH HYDROSTATIC-PRESSURE EFFECTS INVIVO - CHANGES IN CELL MORPHOLOGY, MICROTUBULE ASSEMBLY, AND ACTIN ORGANIZATION
HIGH HYDROSTATIC-PRESSURE EFFECTS INVIVO - CHANGES IN CELL MORPHOLOGY, MICROTUBULE ASSEMBLY, AND ACTIN ORGANIZATION
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DOI：
10.1002/cm.970100305
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发表时间：
1988-01-01
影响因子：
--
通讯作者：
SALMON, ED
中科院分区：
文献类型：
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作者：
BOURNS, B;FRANKLIN, S;SALMON, ED
我们提出了第一个研究的肌动蛋白丝和微管发生在上皮细胞的组装和组织的变化进行深海的静水压力。将间期BSC-1上皮细胞在生理温度下加压并在压力下固定。在细胞形态和细胞骨架组织的变化，随后在1至610大气压的压力范围内。在大气压下，细胞是平坦的并且附着良好。将细胞暴露于290 atm或更大的压力会导致细胞变圆并从基底上缩回。随着压力的增加，这种反应变得更加明显，但在290-400大气压的压力范围内，细胞群内的反应程度不同。微管组装没有明显的影响，压力高达290大气压，但320大气压，很少微管仍然存在。大部分肌动蛋白应力纤维在290 atm时完全消失。高压并不简单地诱导整体解聚的肌动蛋白丝，同时与细胞变圆，可见的微绒毛细胞表面上的数量显着增加。高压的这些影响是可逆的。细胞重新建立其典型的形态，微管阵列出现正常，并在大气压下约1小时后重新形成应力纤维。高压可能会破坏微管和肌动蛋白丝的正常组装，通过影响细胞的调节机制，控制细胞的变化，从间期到有丝分裂的过渡。
We present the first study of the changes in the assembly and organization of actin filaments and microtubules that occur in epithelial cells subjected to the hydrostatic pressures of the deep sea. Interphase BSC-1 epithelial cells were pressurized at physiological temperature and fixed while under pressure. Changes in cell morphology and cytoskeletal organization were followed over a range of pressures from 1 to 610 atm. At atmospheric pressure, cells were flat and well attached. Exposure of cells to pressures of 290 atm or greater caused cell rounding and retraction from the substrate. This response became more pronounced with increased pressure, but the degree of response varied within the cell population in the pressure range of 290-400 atm. Microtubule assembly was not noticeably affected by pressures up to 290 atm, but by 320 atm, few microtubules remained. Most actin stress fibers completely disappeared by 290 atm. High pressure did not simply induce the overall depolymerization of actin filaments for, concurrent with cell rounding, the number of visible microvilli present on the cell surface increased dramatically. These effects of high pressure were reversible. Cells re-established their typical morphology, microtubule arrays appeared normal, and stress fibers reformed after approximately 1 hour at atmospheric pressure. High pressure may disrupt the normal assembly of microtubules and actin filaments by affecting the cellular regulatory mechanisms that control cytological changes during the transition from interphase into mitosis.