ECTOMESENCHYMAL-ENDODERMAL INTERACTION SYSTEM (EEIS) OF TRITURUS-ALPESTRIS IN TISSUE-CULTURE .2. OBSERVATIONS ON DIFFERENTIATION OF VISCERAL CARTILAGE
ECTOMESENCHYMAL-ENDODERMAL INTERACTION SYSTEM (EEIS) OF TRITURUS-ALPESTRIS IN TISSUE-CULTURE .2. OBSERVATIONS ON DIFFERENTIATION OF VISCERAL CARTILAGE
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DOI：
10.1111/j.1432-0436.1975.tb01455.x
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发表时间：
1975-01-01
期刊：
影响因子：
2.9
通讯作者：
LEHMANN, R
中科院分区：
文献类型：
--
作者：
EPPERLEIN, HH;LEHMANN, R
在EEIS中对内脏软骨的分化进行了研究，EEIS是一种悬滴培养系统，包含一块来自高山毛虫神经胚耳部后的头部神经皱褶组织和一块来自同一胚胎的腹外侧咽内胚层。软骨细胞只能从与咽内胚层接触的神经脊细胞分化而来。单独含有神经皱褶或咽内胚层的培养物，或两种外植体彼此之间的距离比平时更远的距离培养时，软骨分化不会发生。在一些含有更多腹侧切除咽内胚层的EEIs培养中，观察到内胚层附近有大量不转化为软骨的外胚间充质细胞。软骨分化分为3个阶段：前软骨母细胞(第1~5天)、软骨母细胞(第6天左右)和软骨细胞(第10天左右)。两个诱导相关组织之间的细胞接触显然是传递机制的先决条件，而有丝分裂细胞分裂显然是诱导信息在反应的外胚间充质细胞内传播的主要原因。
Differentiation of visceral cartilage was studied in EEIS, a hanging-drop culture system containing a piece of head neural fold tissue from behind the prospective ear region of a T. alpestris neurula together with a piece of ventrolateral pharynx endoderm from the same embryo. Cartilage cells differentiated only from those neural crest cells in contact with the pharynx endoderm. Cartilage differentiation did not occur in cultures containing neural fold or pharynx endoderm alone or in distance cultures in which both explants were separated from each other at a greater distance than usual. In some EEIS cultures containing more ventrally excised pharynx endoderm, accumulations of ectomesenchymal cells were observed near the endoderm which were not transformed into cartilage. The differentiation of cartilage was classified into 3 stages: prechondroblasts (days 1-5), chondroblasts (about day 6) and chondrocytes (about day 10). Cellular contact between the 2 inductively correlated tissues was apparently a prerequisite for the transmission mechanism while mitotic cell divisions were evidently mainly responsible for the spread of the inductive message within the responding ectomesenchymal cells.