Dynamic morphoskeletons in development.
Dynamic morphoskeletons in development.
复制标题
发育中的动态形态骨架。
DOI：
10.1073/pnas.1908803117
复制
发表时间：
2020
影响因子：
11.1
通讯作者：
Serra M
中科院分区：
文献类型：
--
作者：
Serra M
发育生物学中的形态发生流以数千个组织成组织的细胞的协调运动为特征，自然地提出了这个集体组织是如何产生的问题。仅使用组织变形的运动学，它自然地整合了沿细胞路径的局部和全局机制，我们识别了形态发生背后的动态形态骨架，即多细胞轨迹模式的进化中心。这些特征与模型和参数无关、帧不变、对测量误差具有健壮性，并且可以从未过滤的细胞速度数据中计算出来。我们通过量化胚胎的拉格朗日变形来揭示胚胎的空间吸引子和排斥子，这是简单的轨迹检查或欧拉方法无法获得的信息，欧拉方法是局部的，通常依赖于框架。对野生型和突变型雏鸟和苍蝇胚胎中的这些动态形态骨架进行计算，我们发现它们捕捉到了已知形态发生特征的早期足迹，揭示了新的特征，并定量区分了不同的表型。
Morphogenetic flows in developmental biology are characterized by the coordinated motion of thousands of cells that organize into tissues, naturally raising the question of how this collective organization arises. Using only the kinematics of tissue deformation, which naturally integrates local and global mechanisms along cell paths, we identify the dynamic morphoskeletons behind morphogenesis, i.e., the evolving centerpieces of multicellular trajectory patterns. These features are model- and parameter-free, frame-invariant, and robust to measurement errors and can be computed from unfiltered cell-velocity data. We reveal the spatial attractors and repellers of the embryo by quantifying its Lagrangian deformation, information that is inaccessible to simple trajectory inspection or Eulerian methods that are local and typically frame-dependent. Computing these dynamic morphoskeletons in wild-type and mutant chick and fly embryos, we find that they capture the early footprint of known morphogenetic features, reveal new ones, and quantitatively distinguish between different phenotypes.